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Clinical and Vaccine Immunology, March 2008, p. 484-491, Vol. 15, No. 3
1071-412X/08/$08.00+0     doi:10.1128/CVI.00415-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Borrelia burgdorferi Complement Regulator-Acquiring Surface Protein 2 (CspZ) as a Serological Marker of Human Lyme Disease{triangledown}

Peter Kraiczy,1,{dagger} Annekatrin Seling,1,{dagger} Catherine A. Brissette,2 Evelyn Rossmann,3 Klaus-Peter Hunfeld,1 Tomasz Bykowski,2,{ddagger} Logan H. Burns,2 Matthew J. Troese,2,§ Anne E. Cooley,2 Jennifer C. Miller,2,¶ Volker Brade,1 Reinhard Wallich,3 Sherwood Casjens,4 and Brian Stevenson2*

Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Frankfurt am Main, Germany,1 Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky,2 Department of Immunology, University of Heidelberg, Heidelberg, Germany,3 Department of Pathology, University of Utah Medical School, Salt Lake City, Utah4

Received 15 October 2007/ Returned for modification 19 November 2007/ Accepted 14 December 2007

Serological diagnosis of Lyme disease may be complicated by antigenic differences between infecting organisms and those used as test references. Accordingly, it would be helpful to include antigens whose sequences are well conserved by a broad range of Lyme disease spirochetes. In the present study, line blot analyses were performed using recombinant complement regulator-acquiring surface protein 2 (BbCRASP-2) from Borrelia burgdorferi sensu stricto strain B31 and serum samples from human Lyme disease patients from throughout the United States and Germany. The results indicated that a large proportion of the patients had produced antibodies recognizing recombinant BbCRASP-2. In addition, Lyme disease spirochetes isolated from across North America and Europe were found to contain genes encoding proteins with high degrees of similarity to the B. burgdorferi type strain B31 BbCRASP-2, consistent with the high percentage of serologically positive patients. These data indicate that BbCRASP-2 may be valuable for use in a widely effective serological assay.

* Corresponding author. Mailing address: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, MS 421, W. R. Willard Medical Education Building, Lexington, KY 40536-0298. Phone: (859) 257-9358. Fax: (859) 257-8994. E-mail: brian.stevenson{at}uky.edu

{triangledown} Published ahead of print on 26 December 2007.

{dagger} P.K. and A.S. contributed equally to the work.

{ddagger} Present address: Center for Medical Education, Warsaw, Poland.

§ Present address: Dept. of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA.

Present address: Department of Pathology, University of Utah Medical School, Salt Lake City, UT.

Clinical and Vaccine Immunology, March 2008, p. 484-491, Vol. 15, No. 3
1071-412X/08/$08.00+0     doi:10.1128/CVI.00415-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rogers, E. A., Abdunnur, S. V., McDowell, J. V., Marconi, R. T. (2009). Comparative Analysis of the Properties and Ligand Binding Characteristics of CspZ, a Factor H Binding Protein, Derived from Borrelia burgdorferi Isolates of Human Origin. Infect. Immun. 77: 4396-4405 [Abstract] [Full Text]  
  • Siegel, C., Schreiber, J., Haupt, K., Skerka, C., Brade, V., Simon, M. M., Stevenson, B., Wallich, R., Zipfel, P. F., Kraiczy, P. (2008). Deciphering the Ligand-binding Sites in the Borrelia burgdorferi Complement Regulator-acquiring Surface Protein 2 Required for Interactions with the Human Immune Regulators Factor H and Factor H-like Protein 1. J. Biol. Chem. 283: 34855-34863 [Abstract] [Full Text]  


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