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Clinical and Vaccine Immunology, December 2008, p. 1764-1770, Vol. 15, No. 12
1071-412X/08/$08.00+0     doi:10.1128/CVI.00270-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Real-Time In Vivo Green Fluorescent Protein Imaging of a Murine Leishmaniasis Model as a New Tool for Leishmania Vaccine and Drug Discovery{triangledown}

Sanjay R. Mehta,1 Robert Huang,1 Meng Yang,2 Xing-Quan Zhang,1 Bala Kolli,3 Kwang-Poo Chang,3 Robert M. Hoffman,2,4 Yasuyuki Goto,5 Roberto Badaro,1,6 and Robert T. Schooley1*

Department of Medicine, University of California, San Diego, San Diego, California,1 AntiCancer Inc., San Diego, California,2 Department of Microbiology/Immunology, Rosalind Franklin University/Chicago Medical School, Chicago, Illinois,3 Department of Surgery, University of California, San Diego, San Diego, California,4 Infectious Diseases Research Institute, Seattle, Washington,5 Federal University of Bahia, Salvador, Brazil6

Received 28 July 2008/ Accepted 13 October 2008

Leishmania species are obligate intracellular protozoan parasites that cause a broad spectrum of clinical diseases in mammalian hosts. The most frequently used approach to quantify parasites in murine model systems is based on thickness measurements of the footpad or ear after experimental infection. To overcome the limitations of this method, we used a Leishmania mutant episomally transfected with enhanced green fluorescent protein, enabling in vivo real-time whole-body fluorescence imaging, to follow the progression of Leishmania infection in parasitized tissues. Fluorescence correlated with the number of Leishmania parasites in the tissue and demonstrated the real-time efficacy of a therapeutic vaccine. This approach provides several substantial advantages over currently available animal model systems for the in vivo study of immunopathogenesis, prevention, and therapy of leishmaniasis. These include improvements in sensitivity and the ability to acquire real-time data on progression and spread of the infection.

* Corresponding author. Mailing address: Stein Clinical Research Bldg. 402A, 9500 Gilman Drive Box 0711, La Jolla, CA 92093. Phone: (858) 822-4092. Fax: (858) 822-5362. E-mail: rschooley{at}ucsd.edu

{triangledown} Published ahead of print on 22 October 2008.

Clinical and Vaccine Immunology, December 2008, p. 1764-1770, Vol. 15, No. 12
1071-412X/08/$08.00+0     doi:10.1128/CVI.00270-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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  • Singh, N., Gupta, R., Jaiswal, A. K., Sundar, S., Dube, A. (2009). Transgenic Leishmania donovani clinical isolates expressing green fluorescent protein constitutively for rapid and reliable ex vivo drug screening. J Antimicrob Chemother 64: 370-374 [Abstract] [Full Text]  


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