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Clinical and Vaccine Immunology, November 2008, p. 1705-1710, Vol. 15, No. 11
1071-412X/08/$08.00+0     doi:10.1128/CVI.00165-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of a Commercial Immunoblot, Helicoblot 2.1, for Diagnosis of Helicobacter pylori Infection {triangledown}

Lea Veijola,1,2* Aino Oksanen,1 Pentti Sipponen,3 and Hilpi Rautelin2,4

Herttoniemi Hospital, City of Helsinki, Helsinki, Finland,1 Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland,2 Biohit PLC Diagnostics, Helsinki, Finland,3 HUSLAB, Helsinki University Central Hospital Laboratory, Helsinki, Finland4

Received 11 May 2008/ Returned for modification 28 July 2008/ Accepted 21 September 2008

The best method to diagnose Helicobacter pylori infection in different clinical situations is controversial. The aim of the study was to assess the performance of a commercial immunoblot, Helicoblot 2.1. The study comprised 215 patients, who were grouped according to the presence of H. pylori infection (assessed by two gastroscopies including histology with a median interval of 7.1 years, enzyme immunoassay [EIA]-based serology, and history of previous H. pylori infections and eradication therapies) into four categories: no H. pylori infection ever, previous infection, ongoing infection, and EIA seropositivity as the only marker of a possible previous infection. The sensitivity of Helicoblot 2.1 to show an ongoing or previous H. pylori infection was 100% and 92%, respectively. Helicoblot 2.1 was negative in only 80% of individuals with no evidence of present or previous infection but in 96% of patients 50 years of age or younger. The current infection marker of the immunoblot was positive in 49% of patients with successful H. pylori eradication therapy. After successful eradication therapy, Helicoblot 2.1 sustained positive results in 87% of patients, and CagA positivity was detected in 87% of patients with follow-up samples for more than 10 years after therapy. Helicoblot 2.1 is a sensitive and, among patients of ages 50 years or younger, a specific test in the primary diagnosis of H. pylori infection. However, it does not discriminate between past and current infections. It can be used in epidemiological studies assessing the role of H. pylori in different late sequelae.

* Corresponding author. Mailing address: Herttoniemi Hospital, Kettutie 8, 00800 Helsinki, Finland. Phone: 358-505564367. Fax: 3589-19126382. E-mail: lea.veijola{at}helsinki.fi

{triangledown} Published ahead of print on 30 September 2008.

Clinical and Vaccine Immunology, November 2008, p. 1705-1710, Vol. 15, No. 11
1071-412X/08/$08.00+0     doi:10.1128/CVI.00165-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gao, L., Michel, A., Weck, M. N., Arndt, V., Pawlita, M., Brenner, H. (2009). Helicobacter pylori Infection and Gastric Cancer Risk: Evaluation of 15 H. pylori Proteins Determined by Novel Multiplex Serology. Cancer Res. 69: 6164-6170 [Abstract] [Full Text]  


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