This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Datta, S.
Right arrow Articles by Simonson, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Datta, S.
Right arrow Articles by Simonson, J. G.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, October 2008, p. 1541-1546, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00141-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Immunomagnetic Separation and Coagglutination of Vibrio parahaemolyticus with Anti-Flagellar Protein Monoclonal Antibody{triangledown}

S. Datta,1 M. E. Janes,1 and J. G. Simonson2*

Department of Food Science,1 Department of Agricultural Chemistry, Louisiana State University AgCenter, Baton Rouge, Louisiana 708032

Received 26 March 2008/ Returned for modification 13 June 2008/ Accepted 15 August 2008

Mice were immunized by injection of Vibrio parahaemolyticus ATCC 17802 polar flagellin in order to produce monoclonal antibodies (mAbs). mAbs were analyzed by anti-H enzyme-linked immunosorbent assay using V. parahaemolyticus polar flagellar cores. The mAb exhibiting the highest anti-H titer was coated onto Cowan I Staphylococcus aureus cells at a concentration of 75 µg/ml cell suspension and used for slide coagglutination. Of 41 isolates identified genetically as V. parahaemolyticus, 100% coagglutinated with the anti-H mAb within 30 s, and the mAb did not react with 30 isolates identified as Vibrio vulnificus. A strong coagglutination reaction with V. parahaemolyticus ATCC 17802 was still observed when the S. aureus cells were armed with as little as 15 µg of mAb/ml S. aureus cell suspension. At this concentration, the mAb cross-reacted with three other Vibrio species, suggesting that they share an identical H antigen or antigens. The anti-H mAb was then used to optimize an immunomagnetic separation protocol which exhibited from 35% to about 45% binding of 102 to 103 V. parahaemolyticus cells in phosphate-buffered saline. The mAb would be useful for the rapid and selective isolation, concentration, and detection of V. parahaemolyticus cells from environmental sources.

* Corresponding author. Mailing address: 102 Agricultural Chemistry Building, Highland Road, LSU, Baton Rouge, LA 70803. Phone: (225) 342-5812. Fax: (225) 342-0027. E-mail: jsimonson{at}agctr.lsu.edu

{triangledown} Published ahead of print on 27 August 2008.

Clinical and Vaccine Immunology, October 2008, p. 1541-1546, Vol. 15, No. 10
1071-412X/08/$08.00+0     doi:10.1128/CVI.00141-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»