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Clinical and Vaccine Immunology, September 2007, p. 1242-1244, Vol. 14, No. 9
1071-412X/07/$08.00+0     doi:10.1128/CVI.00187-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Quality Assurance Program for Peripheral Blood Mononuclear Cell Cryopreservation{triangledown}

Adriana Weinberg,1* Raul Louzao,2 Marisa M. Mussi-Pinhata,3 Maria L. S. Cruz,4 Jorge A. Pinto,5 Maria F. Huff,6 Andrea C. de Castro,7 Maria C. Sucupira,8 and Thomas N. Denny2

University of Colorado Health Sciences Center, Denver, Colorado,1 Duke University, Raleigh-Durham, North Carolina,2 Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil,3 Hospital do Servidor Estadual, Rio de Janeiro, Brazil,4 Universidade de Minas Gerais, Belo Horizonte, MG, Brazil,5 Universidade Federal de Rio de Janeiro, Rio de Janeiro, Brazil,6 Hospital da Conceicão, Porto Alegre, Rio Grande do Sul, Brazil,7 Universidade Federal de São Paulo, São Paulo, Brazil8

Received 4 May 2007/ Returned for modification 6 June 2007/ Accepted 16 July 2007

Seven Brazilian sites participating in the Pediatric AIDS Clinical Trials Group international cryopreservation quality assurance pilot program cryopreserved and shipped peripheral blood mononuclear cells (PBMC) to a central U.S. laboratory for analysis. Cell viability and recovery significantly increased over time. A wet-laboratory training session conducted at the central laboratory significantly improved the quality of the cryopreserved PBMC.

* Corresponding author. Mailing address: 4200 E. 9th Ave., Campus Box C227, Denver, CO 80262. Phone: (303) 315-4624. Fax: (303) 315-1787. E-mail: Adriana.Weinberg{at}uchsc.edu

{triangledown} Published ahead of print on 25 July 2007.

Clinical and Vaccine Immunology, September 2007, p. 1242-1244, Vol. 14, No. 9
1071-412X/07/$08.00+0     doi:10.1128/CVI.00187-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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