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Clinical and Vaccine Immunology, September 2007, p. 1203-1209, Vol. 14, No. 9
1071-412X/07/$08.00+0     doi:10.1128/CVI.00116-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Assessment of Cross-Reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-Cell Epitope Level{triangledown}

H. Martin Vordermeier,1* Jemma Brown,2 Paul J. Cockle,1 Willeke P. J. Franken,3 Sandra M. Arend,3 Tom H. M. Ottenhoff,4 Keith Jahans,2 and R. Glyn Hewinson1

TB Research Group,1 TB Diagnostic Section, Veterinary Laboratories Agency—Weybridge, New Haw, Addlestone KT15 3NB, United Kingdom,2 Department of Infectious Diseases,3 Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands4

Received 13 March 2007/ Returned for modification 29 June 2007/ Accepted 13 July 2007

Cross-reactivity between Mycobacterium kansasii ESAT-6 and CFP-10 homologues and their M. bovis counterparts can confound the interpretation of immunodiagnostic tests for tuberculosis. M. kansasii is a nontuberculous mycobacterial species cultured from skin test-positive cattle in Great Britain. Using peptides derived from M. bovis and M. kansasii ESAT-6 and CFP-10 regions that differ between these species, we investigated the species specificity and cross-reactivity at the level of individual bovine T-cell epitopes. Our results demonstrated that all peptides tested are fully cross-reactive, with the exception of one ESAT-6-derived peptide that harbored an M. bovis-specific epitope(s) when it was recognized in the context of bovine leukocyte antigen (BoLA)-DQ but that was cross-reactive with its M. kansasii homologues when it was restricted by BoLA-DR. This observation further highlights that prediction of species specificity by comparing sequence identity/homology alone is not sufficient and that individuals with diverse major histocompatibility complex constellations need to be tested to characterize the cross-reactivity or species specificity of peptide-based reagents.

* Corresponding author. Mailing address: TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone KT15 3NB, United Kingdom. Phone: 44 1932 357 884. Fax: 44 1932 357 684. E-mail: m.vordermeier{at}vla.defra.gsi.gov.uk

{triangledown} Published ahead of print on 1 August 2007.

Clinical and Vaccine Immunology, September 2007, p. 1203-1209, Vol. 14, No. 9
1071-412X/07/$08.00+0     doi:10.1128/CVI.00116-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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