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Clinical and Vaccine Immunology, September 2007, p. 1094-1101, Vol. 14, No. 9
1071-412X/07/$08.00+0     doi:10.1128/CVI.00183-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Measurement of Staphylococcal Enterotoxin B in Serum and Culture Supernatant with a Capture Enzyme-Linked Immunosorbent Assay{triangledown}

E. Cook,1 X. Wang,1 N. Robiou,2 and B. C. Fries1*

Departments of Medicine, Microbiology, and Immunology, Albert Einstein College of Medicine, Bronx, New York,1 Fordham University, Bronx, New York2

Received 30 April 2007/ Returned for modification 21 June 2007/ Accepted 3 July 2007

Staphylococcal enterotoxin B (SEB) is a select agent because it is a potent mitogen that elicits life-threatening polyclonal T-cell proliferation and cytokine production at very low concentrations. Efforts are in progress to develop therapeutic reagents and vaccines that neutralize or prevent the devastating effects of this toxin. Because of its rapid binding to in vivo receptors, this toxin is difficult to detect in serum. This rapid binding also constitutes a major challenge for the development of effective therapeutic reagents that can neutralize the effects of the toxin in vivo. We have developed a highly sensitive capture enzyme-linked immunosorbent assay that detects SEB in body fluids at very low levels. With this assay, the peak levels of SEB in serum and renal clearance can be measured in mice. After either oral ingestion or nasal inhalation of SEB by mice, this assay documents the transcytosis of SEB across the mucosal membranes into serum within 2 h. Furthermore, this assay was used to compare the SEB levels in different murine models for SEB-induced lethal shock and demonstrated that the coadministration of toxin-enhancing chemicals, such as D-galactosamine and lipopolysaccharide, can alter the peak serum SEB levels. Hence, this assay is a potentially useful tool for the study of the pharmacokinetics of SEB and the effects of potential therapeutic reagents on serum SEB levels.

* Corresponding author. Mailing address: Department of Medicine, Albert Einstein College of Medicine, Ullmann 12233, 1300 Morris Park, Bronx, NY 10461. Phone: (718) 430-2365. Fax: (718) 430-8968. E-mail: fries{at}aecom.yu.edu

{triangledown} Published ahead of print on 18 July 2007.

Clinical and Vaccine Immunology, September 2007, p. 1094-1101, Vol. 14, No. 9
1071-412X/07/$08.00+0     doi:10.1128/CVI.00183-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Varshney, A. K., Mediavilla, J. R., Robiou, N., Guh, A., Wang, X., Gialanella, P., Levi, M. H., Kreiswirth, B. N., Fries, B. C. (2009). Diverse Enterotoxin Gene Profiles among Clonal Complexes of Staphylococcus aureus Isolates from the Bronx, New York. Appl. Environ. Microbiol. 75: 6839-6849 [Abstract] [Full Text]  


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