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Clinical and Vaccine Immunology, June 2007, p. 796-798, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00093-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Extended Culture Enhances Sensitivity of a Gamma Interferon Assay for Latent Mycobacterium tuberculosis Infection{triangledown}

Ana Cehovin,1* Jacqueline M. Cliff,1 Philip C. Hill,2 Roger H. Brookes,2 and Hazel M. Dockrell1

Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom,1 Medical Research Centre, The Fajara, Banjul, The Gambia2

Received 14 February 2007/ Returned for modification 21 March 2007/ Accepted 19 April 2007

To test the hypothesis that prolonged culture would enhance the sensitivity of latent tuberculosis detection by a gamma interferon release assay, blood samples from 33 household contacts of Gambian tuberculosis patients were stimulated with Mycobacterium tuberculosis-specific antigens. After 24 h of culture, 66% were positive, compared to 93% after 6 days of culture.

* Corresponding author. Present address: Department of Cellular and Molecular Medicine, Medical Microbiology, St. George's University of London, Cranmer Terrace, London SW12 0RE, United Kingdom. Phone: 44 20 872 55730. Fax: 44 208 672 0234. E-mail: a.cehovin{at}ucl.ac.uk

{triangledown} Published ahead of print on 25 April 2007.

Clinical and Vaccine Immunology, June 2007, p. 796-798, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00093-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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