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Clinical and Vaccine Immunology, June 2007, p. 726-731, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00456-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein{triangledown}

So-Hee Kim,1 Seung-Young Hwang,2 Yong-Seok Lee,1,2,3 In-Hak Choi,4 Sae-Gwang Park,4,{dagger}* and Weon-Gyu Kho1,2,3,{dagger}*

Department of Malariology, Paik Institute for Clinical Research,1 Department of Parasitology,2 Mitochondrial Research Group, Frontier Inje Research for Science and Technology,3 Department of Microbiology and Center for Viral Disease, College of Medicine, Inje University, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, South Korea4

Received 29 November 2006/ Returned for modification 27 January 2007/ Accepted 12 April 2007

Phage display of single-chain variable fragment (scFv) antibodies is a powerful tool for selecting important, useful, and specific human antibodies. We constructed a library from three patients infected with Plasmodium vivax. Panning on recombinant PvRII enriched a population of scFvs that recognized region II of the P. vivax Duffy binding protein (DBP). Three clones of scFvs that reacted with PvRII were selected, and their biological functions were analyzed. These scFvs inhibited erythrocyte binding to DBP. Clone SFDBII92 had the greatest affinity (dissociation constant = 3.62 x 10–8 M) and the greatest inhibition activity (50% inhibitory concentration {approx} 2.9 µg/ml) to DBP. Thus, we demonstrated that human neutralizing antibody could be made from malaria patients using phage display and that these neutralizing scFvs should prove valuable for developing both passive and active immunization strategies based on DBP.

* Corresponding author. Mailing address for Sae-Gwang Park: Department of Microbiology and Center for Viral Disease Research, College of Medicine, Inje University, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, South Korea. Phone: 82-51-890-6734. Fax: 82-51-891-6004. E-mail: micpsg{at}inje.ac.kr. Mailing address for Weon-Gyu Kho: Department of Malaria Research, Paik Institute for Clinical Research & Department of Parasitology, College of Medicine, Inje University, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, South Korea. Phone: 82-51-890-6731. Fax: 82-51-894-6709. E-mail: wgkho{at}inje.ac.kr

{triangledown} Published ahead of print on 25 April 2007.

{dagger} S.-G.P. and W.-G.K. contributed equally to this work.

Clinical and Vaccine Immunology, June 2007, p. 726-731, Vol. 14, No. 6
1071-412X/07/$08.00+0     doi:10.1128/CVI.00456-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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