This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dos Santos, F. B.
Right arrow Articles by Miagostovich, M. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dos Santos, F. B.
Right arrow Articles by Miagostovich, M. P.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, May 2007, p. 641-643, Vol. 14, No. 5
1071-412X/07/$08.00+0     doi:10.1128/CVI.00474-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Recombinant Polypeptide Antigen-Based Immunoglobulin G Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Dengue{triangledown}

Flavia B. Dos Santos,1* Rita Maria R. Nogueira,1 Monique R. Q. Lima,1 Thatiane S. De Simone,1 Hermann G. Schatzmayr,1 Elezer M. B. Lemes,2 Eva Harris,3 and Marize P. Miagostovich1

Department of Virology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21045-360, Brazil,1 Department of Technological Development, Bio-Manguinhos, Rio de Janeiro 21045-360, Brazil,2 Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720-73603

Received 18 December 2006/ Returned for modification 8 February 2007/ Accepted 8 March 2007

We have developed an indirect enzyme-linked immunosorbent assay for detection of anti-dengue virus (DENV) immunoglobulin G antibodies using four recombinant DENV envelope polypeptides as antigens, which demonstrated a sensitivity of 89.4% and a specificity of 93.3%. These easily produced antigens are a feasible, cost-effective alternative for generating reagents for dengue serological tests.

* Corresponding author. Mailing address: Flavivirus Laboratory-Department of Virology, Oswaldo Cruz Institute/FIOCRUZ, Av Brasil, 4365, Manguinhos, Rio de Janeiro 21045-360, Brazil. Phone and fax: 55(21) 2598-4373. E-mail: flaviab{at}ioc.fiocruz.br

{triangledown} Published ahead of print on 28 March 2007.

Clinical and Vaccine Immunology, May 2007, p. 641-643, Vol. 14, No. 5
1071-412X/07/$08.00+0     doi:10.1128/CVI.00474-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Weissmann, G. (2008). Dengue Fever in Rio: Macumba versus Voltaire. FASEB J. 22: 2109-2112 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»