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Clinical and Vaccine Immunology, May 2007, p. 556-561, Vol. 14, No. 5
1071-412X/07/$08.00+0     doi:10.1128/CVI.00452-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Inadequacy of Colominic Acid as an Absorbent Intended To Facilitate Use of Complement-Preserved Baby Rabbit Serum in the Neisseria meningitidis Serogroup B Serum Bactericidal Antibody Assay{triangledown}

Jamie Findlow,1* Ann Holland,1 Diana Martin,2 Philipp Oster,3 Paul Balmer,1 and Ray Borrow1

Vaccine Evaluation Unit, Health Protection Agency North West, Manchester Laboratory, Manchester Medical Microbiology Partnership, P.O. Box 209, Clinical Sciences Building II, Manchester Royal Infirmary, Manchester M13 9WZ, United Kingdom,1 Institute of Environmental Science & Research Ltd., Kenepuru Science Centre, Porirua, New Zealand,2 Novartis Vaccines, Via Fiorentina 1, 53100 Siena, Italy3

Received 4 December 2006/ Returned for modification 11 January 2007/ Accepted 27 February 2007

The surrogate of protection against Neisseria meningitidis serogroup B (MenB) is the serum bactericidal antibody (SBA) assay, which measures the functional activity of antibody by using an exogenous complement source. Despite baby rabbit complement having been used in meningococcal serogroup A, C, Y, and W135 SBA assays, it is not recommended for use in the MenB SBA assay due to elevated SBA titers caused by low-avidity anti-MenB capsular antibody in test sera. Therefore, the possibility of absorbing anti-MenB capsular antibody from test sera to enable the use of baby rabbit complement in the MenB SBA assay was investigated by comparing the results with those gained using human complement. Colominic acid from Escherichia coli K1, which shares the same linkage residue as MenB polysaccharide, was used as an absorbent due to the commercial unavailability of purified MenB polysaccharide. Inclusion of soluble colominic acid as an absorbent with baby rabbit complement resulted in a general reduction in SBA titers compared with those obtained using baby rabbit complement alone. However, these were not comparable to human SBA titers for all samples. Further optimization and investigations demonstrated that for some samples, colominic acid reduced titers to less than those achieved with human complement, and for others, it was not possible to inhibit titers by using colominic acid. The results suggested that the use of colominic acid will not result in the ability to use baby rabbit complement in the MenB SBA assay, thus not alleviating the difficulties in procuring human complement. However, alternative absorbents, such as purified MenB polysaccharide, may warrant further evaluation.

* Corresponding author. Mailing address: Vaccine Evaluation Unit, Health Protection Agency North West, Manchester Laboratory, Manchester Medical Microbiology Partnership, P.O. Box 209, Clinical Sciences Building II, Manchester Royal Infirmary, Manchester M13 9WZ, United Kingdom. Phone: 44 (0) 161 276 6791. Fax: 44 (0) 161 276 6792. E-mail: jamie.findlow{at}hpa.org.uk

{triangledown} Published ahead of print on 7 March 2007.

Clinical and Vaccine Immunology, May 2007, p. 556-561, Vol. 14, No. 5
1071-412X/07/$08.00+0     doi:10.1128/CVI.00452-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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