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Clinical and Vaccine Immunology, February 2007, p. 201-203, Vol. 14, No. 2
1071-412X/07/$08.00+0     doi:10.1128/CVI.00223-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of Dried Whole Blood Spots Obtained by Heel or Finger Stick as an Alternative to Venous Blood for Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Vertically Exposed Infants in the Routine Diagnostic Laboratory{triangledown}

Janet C. Patton,1 Eveline Akkers,2,4 Ashraf H. Coovadia,1,3 Tammy M. Meyers,1,3 Wendy S. Stevens,2,4 and Gayle G. Sherman1,2,4*

Wits Pediatric HIV Clinics, Wits Health Consortium,1 Departments of Molecular Medicine and Hematology,2 Pediatrics, University of the Witwatersrand, Johannesburg, Gauteng, South Africa,3 National Health Laboratory Service, Johannesburg, Gauteng, South Africa4

Received 15 June 2006/ Returned for modification 20 September 2006/ Accepted 1 December 2006

The diagnostic accuracy of the Roche Amplicor human immunodeficiency virus type 1 DNA PCR assay (version 1.5) on DNA extracted from pediatric heel prick dried blood spots using Roche MagNA Pure nucleic acid purification technology was evaluated. The methodologies transfer successfully from the labor-intensive research laboratory to the high-throughput automated routine laboratory.

* Corresponding author. Mailing address: P.O. Box 79722 Senderwood, 2145 Johannesburg, South Africa. Phone: 27 11 488 3693. Fax: 27 11 489 8589. E-mail: gayle.sherman{at}nhls.ac.za.

{triangledown} Published ahead of print on 13 December 2006.

Clinical and Vaccine Immunology, February 2007, p. 201-203, Vol. 14, No. 2
1071-412X/07/$08.00+0     doi:10.1128/CVI.00223-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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