This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Denis, M.
Right arrow Articles by Buddle, B. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Denis, M.
Right arrow Articles by Buddle, B. M.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, November 2007, p. 1483-1489, Vol. 14, No. 11
1071-412X/07/$08.00+0     doi:10.1128/CVI.00291-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Enhancement of the Sensitivity of the Whole-Blood Gamma Interferon Assay for Diagnosis of Mycobacterium bovis Infections in Cattle{triangledown}

Michel Denis,1 D. Neil Wedlock,1 Allison R. McCarthy,1 Natalie A. Parlane,1 Paul J. Cockle,2 H. Martin Vordermeier,2 R. Glyn Hewinson,2 and Bryce M. Buddle1*

AgResearch, Hopkirk Research Institute, Palmerston North, New Zealand,1 Veterinary Laboratory Agency, Weybridge, Surrey, United Kingdom2

Received 17 July 2007/ Returned for modification 5 September 2007/ Accepted 7 September 2007

In this study, we determined if the sensitivity of the currently available in vitro test to detect bovine tuberculosis could be enhanced by adding the following immunomodulators: interleukin-2 (IL-2); granulocyte-macrophage colony-stimulating factor (GM-CSF); antibodies neutralizing IL-10 and transforming growth factor ß (TGF-ß); mono-methyl-L-arginine, which blocks nitric oxide production; and L-methyl-tryptophan, which interferes with the indoleamine dioxygenase pathway. Blood was obtained from uninfected control cattle, experimentally infected cattle, cattle responding positively to the skin test in tuberculosis-free areas (false positives), and cattle naturally infected with Mycobacterium bovis from New Zealand and Great Britain. Gamma interferon (IFN-{gamma}) responses to bovine purified protein derivative (PPD-b), avian purified protein derivative, and a fusion protein of ESAT-6 and CFP-10 were measured. Mono-methyl-L-arginine, L-methyl-tryptophan, or an antibody neutralizing TGF-ß had minimal impact on IFN-{gamma} production. IL-2 and GM-CSF promoted IFN-{gamma} release whether antigen was present or not. In contrast, adding an antibody against IL-10 enhanced only antigen-specific responses. In particular, addition of anti-IL-10 to ESAT-6/CFP-10-stimulated blood cultures enhanced the test sensitivity. Furthermore, whole blood cells from field reactors produced substantial amounts of IL-10 upon stimulation with PPD-b or ESAT-6/CFP-10. Testing "false-positive" cattle from tuberculosis-free areas of New Zealand revealed that addition of anti-IL-10 did not compromise the test specificity. Therefore, the use of ESAT-6/CFP-10 with anti-IL-10 could be useful to detect cattle potentially infected with tuberculosis, which are not detected using current procedures.

* Corresponding author. Mailing address: AgResearch, Hopkirk Research Institute, Palmerston North, New Zealand. Phone: 64-6-351-8679. Fax: 64-6-353-7853. E-mail: bryce.buddle{at}agresearch.co.nz

{triangledown} Published ahead of print on 19 September 2007.

Clinical and Vaccine Immunology, November 2007, p. 1483-1489, Vol. 14, No. 11
1071-412X/07/$08.00+0     doi:10.1128/CVI.00291-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lybeck, K. R., Storset, A. K., Olsen, I. (2009). Neutralization of Interleukin-10 from CD14+ Monocytes Enhances Gamma Interferon Production in Peripheral Blood Mononuclear Cells from Mycobacterium avium subsp. paratuberculosis-Infected Goats. CVI 16: 1003-1011 [Abstract] [Full Text]  
  • Ruhwald, M., Bodmer, T., Maier, C., Jepsen, M., Haaland, M. B., Eugen-Olsen, J., Ravn, P., on behalf of TBNET, (2008). Evaluating the potential of IP-10 and MCP-2 as biomarkers for the diagnosis of tuberculosis. Eur Respir J 32: 1607-1615 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»