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Clinical and Vaccine Immunology, November 2007, p. 1458-1464, Vol. 14, No. 11
1071-412X/07/$08.00+0     doi:10.1128/CVI.00482-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Prospective Study To Determine Accuracy of Rapid Serological Assays for Diagnosis of Acute Dengue Virus Infection in Laos{triangledown}

Stuart D. Blacksell,1,2,3* David Bell,4 James Kelley,4 Mammen P. Mammen Jr.,5 Robert V. Gibbons,5 Richard G. Jarman,5 David W. Vaughn,5,6 Kemajittra Jenjaroen,3 Ananda Nisalak,5 Soulignasack Thongpaseuth,1 Manivanh Vongsouvath,1 Viengmone Davong,1 Phonelavanh Phouminh,1 Rattanaphone Phetsouvanh,1 Nicholas P. J. Day,1,2,3 and Paul N. Newton1,2*

Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR,1 Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom,2 Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand,3 World Health Organization Regional Office for the Western Pacific, Manila, Philippines,4 Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand,5 Military Infectious Diseases Research Program, U.S. Army Medical Research and Materiel Command, Fort Detrick, Maryland6

Received 23 December 2006/ Returned for modification 14 May 2007/ Accepted 9 August 2007

There is an urgent need for accurate and simple dengue virus infection diagnostic assays in limited-resource settings of dengue endemicity, to assist patient management. Using a panel of reference samples (S. D. Blacksell, P. N. Newton, D. Bell, J. Kelley, M. P. Mammen, D. W. Vaughn, V. Wuthiekanun, A. Sungkakum, A. Nisalak, and N. P. Day, Clin. Infect. Dis. 42:1127-1134, 2006), we recently evaluated eihgt commercially available immunochromatographic rapid diagnostic tests (RDTs) designed to detect dengue virus-specific immunoglobulin M (IgM) and/or IgG. We found that 6/8 RDTs had sensitivities of less than 50% (range, 6 to 65%), but specificities were generally high. Here, in conjuction with dengue virus serotyping by reverse transcriptase PCR and in the limited-resource setting of Laos, where dengue virus is endemic, we evaluated the same eight RDTs against a previously validated dengue IgM/IgG enzyme-linked immunosorbent assay for diagnosis of acute dengue virus infection. Paired serum samples were collected from 87 patients, of whom 38 had confirmed dengue virus infections (4 had primary infections, 33 had secondary infections, and 1 had an infection of indeterminate status). RDT sensitivity was low, with 7/8 RDTs having admission sample sensitivities of less than 20% (range, 4 to 26%). The majority (6/8) of the RDTs, demonstrated high specificity (>95%). Kappa statistic values ranged from 6 to 54% for the RDTs, demonstrating poor to moderate variation between three operators. No RDT adequately differentiated between primary and secondary dengue virus infections. The findings of this study suggest that currently available RDTs based on the detection of IgM antibodies for the diagnosis of acute dengue virus infections are unlikely to be useful for patient management.

* Corresponding author. Mailing address for Paul N. Newton: Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR. Phone and fax: 856 21 242168. E-mail: paul{at}tropmedres.ac. Mailing address for Stuart D. Blacksell: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. Phone: 66 2 3549172. Fax: 66 2 3549169. E-mail: stuart{at}tropmedres.ac

{triangledown} Published ahead of print on 22 August 2007.

Clinical and Vaccine Immunology, November 2007, p. 1458-1464, Vol. 14, No. 11
1071-412X/07/$08.00+0     doi:10.1128/CVI.00482-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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