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Clinical and Vaccine Immunology, January 2007, p. 99-101, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00136-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Naturally Occurring Anti-Escherichia coli Protein Antibodies in the Sera of Healthy Humans Cause Analytical Interference in a Recombinant Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Severe Acute Respiratory Syndrome{triangledown}

Chi Wai Yip,1 Chung Chau Hon,1 Fanya Zeng,1 Ken Y. C. Chow,1 Kwok Hung Chan,2 Joseph S. M. Peiris,2 and Frederick C. C. Leung1*

Department of Zoology,1 Department of Microbiology, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, China2

Received 11 April 2006/ Returned for modification 25 July 2006/ Accepted 6 November 2006

We reported the analytical interference of anti-Escherichia coli protein (EP) antibodies in human sera and residual EP in a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay as a possible source of false positives in severe acute respiratory syndrome serodiagnosis. The rate of false positives was significantly reduced by adding mouse anti-EP antiserum in the blocking step.

* Corresponding author. Mailing address: Department of Zoology, Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, China. Phone: (852) 2299-0825. Fax: (852) 2857-4672. E-mail: fcleung{at}hkucc.hku.hk.

{triangledown} Published ahead of print on 15 November 2006.

Clinical and Vaccine Immunology, January 2007, p. 99-101, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00136-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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