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Clinical and Vaccine Immunology, January 2007, p. 87-89, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00272-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Performance of a Commercial Immunoglobulin M Antibody Capture Assay Using Analyte-Specific Reagents To Screen for Interfering Factors during a West Nile Virus Epidemic Season in Nebraska{triangledown}

Anthony R. Sambol,1* Steven H. Hinrichs,1 Wayne R. Hogrefe,2 and Beth K. Schweitzer1

Nebraska Public Health Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska,1 Focus Diagnostics, 5785 Corporate Ave., Cypress, California 906302

Received 26 July 2006/ Accepted 7 November 2006

In 2003, the Nebraska Public Health Laboratory tested more than 10,371 serum and 516 cerebral spinal fluid specimens. Results showed that without performing the interfering factors screen for specimens in the low positive index value range of >1.1 to ≤3.5, a false positivity rate of 6.5% would have been realized.

* Corresponding author. Mailing address: Nebraska Public Health Laboratory, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198-6495. Phone: (402) 559-3032. Fax: (402) 559-7799. E-mail: asambol{at}unmc.edu.

{triangledown} Published ahead of print on 22 November 2006.

Clinical and Vaccine Immunology, January 2007, p. 87-89, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00272-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rawlins, M. L., Swenson, E. M., Hill, H. R., Litwin, C. M. (2007). Evaluation of an Enzyme Immunoassay for Detection of Immunoglobulin M Antibodies to West Nile Virus and the Importance of Background Subtraction in Detecting Nonspecific Reactivity. CVI 14: 665-668 [Abstract] [Full Text]  


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