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Clinical and Vaccine Immunology, January 2007, p. 60-64, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00244-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Human Prolactin Promotes Human Secondary Immunoglobulin Response in Human/SCID Mouse Chimeras{triangledown}

Jian Zhang,1* Rui Sun,2 and Zhigang Tian1*

Institute of Immunopharmacology & Immunotherapy, School of Pharmaceutical Sciences, Shandong University, 44 Wenhua West Road, Jinan 250012, China,1 Institute of Life Science, University of Science & Technology of China, 443 Huangshan Road, Hefei 230027, China2

Received 5 July 2006/ Returned for modification 12 September 2006/ Accepted 19 October 2006

Recombinant human prolactin (rhPRL) was administered to huPBL-SCID mice to determine its effects on production of human immunoglobulin (Ig). The huPBL-SCID mice were injected intraperitoneally (i.p.) with 10 µg rhPRL every other day for a total of 10 injections. The results reconfirmed that rhPRL significantly increased the numbers of human CD3+ T cells and human CD19+ B cells in spleens, lymph nodes, and thymuses of huPBL-SCID mice. The huPBL-SCID mice were then concurrently given various doses of diphtheria-tetanus (DT) vaccine and 10-µg i.p. injections of rhPRL and were examined for the presence of human DT-specific proliferation of lymph node cells in vitro and antibody production in vivo. rhPRL greatly improved the engraftment of functional human lymphocytes (CD3+ T cells and CD19+ B cells) in DT-immunized huPBL-SCID mice. The rhPRL-treated, DT-immunized huPBL-SCID mice produced significantly larger amounts of DT-specific antibodies in response to the vaccine. The predominant Ig isotype induced after immunization was IgG. Thus, rhPRL stimulation promotes human secondary IgG responses in huPBL-SCID mice.

* Corresponding author. Mailing address: Institute of Immunopharmacology & Immunotherapy, School of Pharmaceutical Sciences, Shandong University, 44 Wenhua West Road, Jinan 250012, China. Phone: 86-531-88381980. Fax: 86-531-88383782. E-mail for Jian Zhang: zhangj65{at}sdu.edu.cn. E-mail for Zhigang Tian: tzg{at}ustc.edu.cn.

{triangledown} Published ahead of print on 1 November 2006.

Clinical and Vaccine Immunology, January 2007, p. 60-64, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00244-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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