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Clinical and Vaccine Immunology, January 2007, p. 102-105, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00138-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Rapid Expression of Mycobacterium avium subsp. paratuberculosis Recombinant Proteins for Antigen Discovery{triangledown}

Lingling Li,1 Shirin Munir,1 John P. Bannantine,2 Srinand Sreevatsan,3 Sagarika Kanjilal,4 and Vivek Kapur1*

Department of Microbiology and Biomedical Genomics Center,1 Department of Veterinary Population Medicine,3 Department of Medicine, University of Minnesota, St. Paul, Minnesota,4 National Animal Disease Center-USDA-ARS, Ames, Iowa2

Received 1 April 2006/ Returned for modification 8 May 2006/ Accepted 19 October 2006

Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of ruminants and other species. Detection of infection in animals is hampered by the lack of sensitive and specific diagnostic assays. We describe here an approach that utilizes translationally active PCR fragments for the rapid in vitro transcription and translation of recombinant proteins for antigen discovery in M. avium subsp. paratuberculosis. The investigations showed that the MAP1272c protein selectively reacts with sera from Johne's disease-positive cattle and represents an antigen of potential utility in M. avium subsp. paratuberculosis immunodiagnostics.

* Corresponding author. Mailing address: Department of Microbiology, University of Minnesota, 1500 Gortner Ave., St. Paul, MN 55108. Phone: (612) 625-7712. Fax: (612) 624-6264. E-mail: vkapur{at}umn.edu.

{triangledown} Published ahead of print on 1 November 2006.

Clinical and Vaccine Immunology, January 2007, p. 102-105, Vol. 14, No. 1
1071-412X/07/$08.00+0     doi:10.1128/CVI.00138-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Begg, D. J., de Silva, K., Bosward, K., Di Fiore, L., Taylor, D. L., Jungersen, G., Whittington, R. J. (2009). Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease. jvdi 21: 187-196 [Abstract] [Full Text]  
  • Bannantine, J. P., Paustian, M. L., Waters, W. R., Stabel, J. R., Palmer, M. V., Li, L., Kapur, V. (2008). Profiling Bovine Antibody Responses to Mycobacterium avium subsp. paratuberculosis Infection by Using Protein Arrays. Infect. Immun. 76: 739-749 [Abstract] [Full Text]  


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