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Clinical and Vaccine Immunology, August 2006, p. 966-968, Vol. 13, No. 8
1071-412X/06/$08.00+0     doi:10.1128/CVI.00102-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A Novel Double-Antigen Sandwich Enzyme-Linked Immunosorbent Assay for Measurement of Antibodies against Rabies Virus

Li-Min Yang,1 Liang-Zhen Zhao,1,2 Rong-Liang Hu,3 Zhen-Sheng Shi,4 and Wen-Jun Liu1*

The Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China,1 College of Life Science, Henan Normal University, Xinxiang, Henan Province, China,2 Institute of Military Veterinary, Military Medical Scientific Academy, Changchun, Jilin Province, China,3 College of Veterinary Medicine, China Agricultural University, Beijing, China4

Received 14 March 2006/ Returned for modification 20 April 2006/ Accepted 5 June 2006

A novel double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) was developed to measure rabies antibodies in dogs. In contrast to the 4 days required for detecting rabies antibody with conventional rabies antibody virus neutralization assays, this ELISA can be completed in hours, without using live virus, in routine laboratories.

* Corresponding author. Mailing address: The Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, No. 13 Beiyitio, Zhongguancun, Beijing, China. Phone: 86 10 6255 7874. Fax: 86 10 6263 8846. E-mail: liuwj{at}im.ac.cn.

Clinical and Vaccine Immunology, August 2006, p. 966-968, Vol. 13, No. 8
1071-412X/06/$08.00+0     doi:10.1128/CVI.00102-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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