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Clinical and Vaccine Immunology, August 2006, p. 869-875, Vol. 13, No. 8
1071-412X/06/$08.00+0     doi:10.1128/CVI.00103-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mycobacterium tuberculosis HBHA Protein Reacts Strongly with the Serum Immunoglobulin M of Tuberculosis Patients

A-Rum Shin,1 Kil-Soo Lee,1 Ji-Sook Lee,2 Su-Young Kim,1 Chang-Hwa Song,1 Saet-Byel Jung,1 Chul-Su Yang,1 Eun-Kyeong Jo,1 Jeong-Kyu Park,1 Tae-Hyun Paik,2 and Hwa-Jung Kim1*

Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 301-747,1 Department of Microbiology, College of Medicine, Konyang University, Nonsan, Chungnam 320-711, South Korea2

Received 27 February 2006/ Returned for modification 4 May 2006/ Accepted 12 June 2006

Identification and characterization of serologically active mycobacterial antigens are prerequisites for the development of diagnostic reagents. We examined the humoral immune responses of active tuberculosis (TB) patients against Triton-soluble proteins extracted from Mycobacterium tuberculosis by immunoblotting. A 29-kDa protein reacted with immunoglobulin M (IgM) in the pooled sera of the patients, and its N-terminal amino acid sequence matched that of the heparin-binding hemagglutinin (HBHA). Recombinant full-length HBHA was expressed in Escherichia coli (rEC-HBHA) and M. smegmatis (rMS-HBHA). In immunoblot analysis, the IgM antibodies of the TB patients reacted strongly with rMS-HBHA but not with rEC-HBHA, whereas the IgG antibodies of these patients reacted weakly with both recombinant HBHA proteins. In enzyme-linked immunosorbent assay analysis using rMS-HBHA and 85B as antigens, the mean levels and sensitivities of the anti-HBHA IgM antibodies of the TB patients were significantly higher than those of the anti-antigen 85B IgM antibodies, while the IgG antibodies showed the opposite results. Of interest in this respect, the pooled sera from the TB patients that contained anti-HBHA IgM antibodies neutralized the entry of M. tuberculosis into epithelial cells. These findings suggest that IgM antibody to HBHA may play a role in protection against extrapulmonary dissemination.

* Corresponding author. Mailing address: Department of Microbiology, College of Medicine, Chungnam National University, 6 Muwha-Dong, Jung-Ku, Daejeon 301-747, Korea. Phone: 82-42-580-8242. Fax: 82-42-585-3686. E-mail: hjukim{at}cnu.ac.kr.

Clinical and Vaccine Immunology, August 2006, p. 869-875, Vol. 13, No. 8
1071-412X/06/$08.00+0     doi:10.1128/CVI.00103-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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