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Clinical and Vaccine Immunology, June 2006, p. 708-710, Vol. 13, No. 6
1071-412X/06/$08.00+0     doi:10.1128/CVI.00413-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison of PCR for Sputum Samples Obtained by Induced Cough and Serological Tests for Diagnosis of Mycoplasma pneumoniae Infection in Children

Tsutomu Yamazaki,1* Mitsuo Narita,2 Nozomu Sasaki,1 Tsuyoshi Kenri,3 Yoshichika Arakawa,3 and Tsuguo Sasaki3

Department of Pediatrics, Saitama Medical School, Saitama,1 Department of Pediatrics, Sapporo Tetsudo (JR) Hospital, Hokkaido,2 Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan3

Received 30 November 2005/ Returned for modification 9 January 2006/ Accepted 24 March 2006

Passive agglutination (PA) and immunoglobulin M (IgM), IgA, and IgG enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of Mycoplasma pneumoniae were compared with PCR testing of sputum samples obtained from children with lower respiratory tract infections. The sensitivity and specificity of PA were 80.3% and 92.3% at a titer of 1:80. ELISA was found to be less sensitive than PA.

* Corresponding author. Mailing address: Department of Pediatrics, Saitama Medical School, Morohongo 38, Moroyama, Iruma, Saitama, 350-0495 Japan. Phone and fax: 81-49-276-1220. E-mail: benyama{at}saitama-med.ac.jp.

Clinical and Vaccine Immunology, June 2006, p. 708-710, Vol. 13, No. 6
1071-412X/06/$08.00+0     doi:10.1128/CVI.00413-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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