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Clinical and Vaccine Immunology, May 2006, p. 553-555, Vol. 13, No. 5
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.5.553-555.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Immunochromatographic Test for Simultaneous Serodiagnosis of Babesia caballi and B. equi Infections in Horses

Xiaohong Huang,1,2 Xuenan Xuan,1 Rodolfo A. Verdida,1 Shoufa Zhang,3 Naoaki Yokoyama,1 Longshan Xu,2 and Ikuo Igarashi1*

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan,1 Fujian Provincial Center for Diseases Control and Prevention, Fuzhou, Fujian Province 350001, China,2 Department of Veterinary Medicine, Yanbian University, Longjing, Jilin Province 133400, China3

Received 6 December 2005/ Returned for modification 30 January 2006/ Accepted 6 March 2006

An immunochromatographic test for the simultaneous detection of Babesia caballi- and B. equi-specific antibodies (BceICT) was developed using a recombinant B. caballi 48-kDa rhoptry protein (rBc48) and a recombinant truncated B. equi merozoite antigen 2 (rEMA-2t). An evaluation of the ability of the BceICT to detect antibodies in sera from uninfected horses and experimentally infected horses showed high sensitivities and specificities of 83.3% (10/12 sera) and 92.9% (52/56 sera), respectively, for the anti-B. caballi antibody and 94.1% (16/17 sera) and 88.2% (45/51 sera), respectively, for the anti-B. equi antibody. Results from the detection of antibodies in field-collected sera indicated that the BceICT results corresponded with those of enzyme-linked immunosorbent assays (ELISA), showing 91.8% correspondence (67/73 sera) for B. caballi and 95.9% correspondence (70/73 sera) for B. equi, and that the BceICT results also corresponded with the ICT for B. caballi and for B. equi, both of which were 98.2% (55/56 sera). The comparable results of the ICT and ELISA and the simplicity and rapidity of the performance of the ICT suggest that the BceICT would be a feasible test for the simultaneous serodiagnosis of both agents of equine babesiosis in the field.

* Corresponding author. Mailing address: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Phone: 81-155-49-5641. Fax: 81-155-49-5643. E-mail: igarcpmi{at}obihiro.ac.jp.

Clinical and Vaccine Immunology, May 2006, p. 553-555, Vol. 13, No. 5
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.5.553-555.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kim, C.-M., Blanco, L. B. C., Alhassan, A., Iseki, H., Yokoyama, N., Xuan, X., Igarashi, I. (2008). Development of a Rapid Immunochromatographic Test for Simultaneous Serodiagnosis of Bovine Babesioses Caused by Babesia bovis and Babesia bigemina. Am J Trop Med Hyg 78: 117-121 [Abstract] [Full Text]  


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