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Clinical and Vaccine Immunology, March 2006, p. 420-422, Vol. 13, No. 3
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.3.420-422.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

S. E. Burastero,1* C. Paolucci,1 D. Breda,1 G. Monasterolo,2 R. E. Rossi,3 and L. Vangelista1,4

San Raffaele Scientific Institute, Milan,1 Laboratorio Analisi Ospedale S.S. Trinità, Fossano (Cuneo),2 SSN Regione Piemonte, Savigliano (Cuneo),3 Department of Biology and Genetics, University of Milan, Milan, Italy4

Received 12 October 2005/ Returned for modification 8 December 2005/ Accepted 9 January 2006

The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fc{varepsilon}RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

* Corresponding author. Mailing address: San Raffaele Scientific Institute, via Olgettina 58, 20132 Milan, Italy. Phone: 390226434730. Fax: 390226434723. E-mail: s.burastero{at}hsr.it.

Clinical and Vaccine Immunology, March 2006, p. 420-422, Vol. 13, No. 3
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.3.420-422.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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