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Clinical and Vaccine Immunology, March 2006, p. 361-364, Vol. 13, No. 3
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.3.361-364.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of Vircell Enzyme-Linked Immunosorbent Assay and Indirect Immunofluorescence Assay for Detection of Antibodies against Legionella pneumophila

Bram M. W. Diederen,1* Jan A. J. W. Kluytmans,2 and Marcel F. Peeters1

Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, P.O. Box 747, 5000 AS Tilburg, The Netherlands,1 Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands2

Received 3 August 2005/ Returned for modification 13 September 2005/ Accepted 13 January 2006

We evaluated the abilities of the Vircell immunoglobulin G (IgG) and IgM indirect immunofluorescence assay (IFA) for Legionella pneumophila serogroup 1, the IgM and IgG enzyme-linked immunosorbent assay (ELISA) for Legionella pneumophila serogroup 1, and the IgM-plus-IgG ELISA for Legionella pneumophila serogroups 1 to 6 to diagnose Legionnaires' disease (LD) in a well-described sample of patients with and without LD. Also, we determined the agreements, sensitivities, and specificities of the different Vircell assays in comparison to a validated ELISA (Serion classic ELISA). Clinical sensitivity and specificity were 74.6% and 96.6%, respectively, for the IgM IFA, 65.1% and 88.0% for the IgG IFA, 92.3% and 100% for the IgM ELISA, 43.3% and 96.6% for the IgG ELISA, and 90.8% and 100% for the IgM-plus-IgG ELISA. Compared to Serion classic ELISA, agreement, sensitivity, and specificity were 80.0%, 83.1%, and 78.4%, respectively, for the IgM IFA, 75.2%, 66.0%, and 79.5% for the IgG IFA, 89.5%, 82.0%, and 97.6% for the IgM ELISA, 81.9%, 88.9%, and 78.0% for the IgG ELISA, and 93.5%, 90.0%, and 96.6% for the IgM-plus-IgG ELISA. The value of a positive diagnostic result obtained by the Vircell IgM IFA, the Vircell IgG IFA, and the Vircell IgG ELISA might not be acceptable for a diagnostic assay. Both the high specificities and sensitivities of the Vircell IgM ELISA and the IgM-plus-IgG ELISA and the high correlation with the Serion classic ELISA indicate that they are useful in the diagnosis of LD.

* Corresponding author. Mailing address: Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, P.O. Box 747, 5000 AS Tilburg, The Netherlands. Phone: 31 13 539 2655. Fax: 31 13 544 1264. E-mail: b.diederen{at}elisabeth.nl.

Clinical and Vaccine Immunology, March 2006, p. 361-364, Vol. 13, No. 3
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.3.361-364.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rojas, A., Rojas, J., Mendoza, J., Diederen, B. M. W., Peeters, M. F., Kluytmans, J. A. J. W. (2007). Vircell Assays for Detection of Antibodies against Legionella pneumophila. CVI 14: 208-209 [Full Text]  


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