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Clinical and Vaccine Immunology, December 2006, p. 1363-1366, Vol. 13, No. 12
1071-412X/06/$08.00+0     doi:10.1128/CVI.00239-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Diagnosis of Paracoccidioidomycosis by Detection of Antigen and Antibody in Bronchoalveolar Lavage Fluids{triangledown}

Silvia Helena Marques-da-Silva,1 Arnaldo Lopes Colombo,2 Maria Heloisa Souza Lima Blotta,3 Flávio Queiroz-Telles,4 Alípio Barbosa Balthazar,5 José Daniel Lopes,1 and Zoilo Pires de Camargo1*

Department of Microbiology, Immunology, and Parasitology, Federal University of São Paulo (UNIFESP), São Paulo, Brazil,1 Division of Infectious Diseases, Federal University of São Paulo (UNIFESP), São Paulo, Brazil,2 Department of Clinical Pathology,3 Department of Internal Medicine, School of Medicine, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil,5 Department of Community Health, School of Medicine, Federal University of Paraná, Curitiba, Paraná, Brazil4

Received 29 June 2006/ Returned for modification 22 August 2006/ Accepted 2 October 2006

Paracoccidioidomycosis (PCM) is a systemic infection caused by the fungus Paracoccidioides brasiliensis and is believed to be the leading cause of fungal pulmonary infection. In this study, we used an inhibition enzyme-linked immunosorbent assay to diagnose pulmonary PCM based on the detection of 43-kDa and 70-kDa molecules in bronchoalveolar lavage fluids. The results were compared with results obtained by classical methods for antibody detection.

* Corresponding author. Mailing address: Universidade Federal de São Paulo (UNIFESP), Disciplina de Biologia Celular, 04023-062, Rua Botucatu, 862, 8° andar, São Paulo, SP, Brazil. Phone: 55 11 5576 4523. Fax: 55 11 5571 5877. E-mail: zoilo{at}ecb.epm.br.

{triangledown} Published ahead of print on 11 October 2006.

Clinical and Vaccine Immunology, December 2006, p. 1363-1366, Vol. 13, No. 12
1071-412X/06/$08.00+0     doi:10.1128/CVI.00239-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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