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Clinical and Vaccine Immunology, October 2006, p. 1166-1169, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00219-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Limited Diagnostic Capacities of Two Commercial Assays for the Detection of Leptospira Immunoglobulin M Antibodies in Laos

Stuart D. Blacksell,1,2,3* Lee Smythe,4 Rattanaphone Phetsouvanh,1 Michael Dohnt,4 Rudy Hartskeerl,5 Meegan Symonds,4 Andrew Slack,4 Manivanh Vongsouvath,1 Viengmone Davong,1 Olay Lattana,1 Simmaly Phongmany,1 Valy Keolouangkot,1 Nicholas J. White,1,2,3 Nicholas P. J. Day,1,2,3 and Paul N. Newton1,2*

Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic,1 Centre for Clinical Vaccinology and Tropical Medicine, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, United Kingdom,2 Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Rd., Bangkok 10400, Thailand,3 WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, Coopers Plains, Queensland, 4108, Australia,4 WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, KIT Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands5

Received 2 June 2006/ Returned for modification 11 July 2006/ Accepted 31 July 2006

The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira "gold standard" microscopic agglutination test.

* Corresponding authors. Mailing address for P. Newton: Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR. Phone: (856) 21 242168. Fax: (856) 21 242168. E-mail: paul{at}tropmedres.ac. Mailing address for S. Blacksell: Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Rd., Bangkok 10400, Thailand. Phone: (66) 2 354 9171. Fax: (66) 2 354 9169. E-mail: stuart{at}tropmedres.ac.

Clinical and Vaccine Immunology, October 2006, p. 1166-1169, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00219-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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