This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cockle, P. J.
Right arrow Articles by Vordermeier, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cockle, P. J.
Right arrow Articles by Vordermeier, H. M.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, October 2006, p. 1119-1124, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00209-06

Field Evaluation of a Novel Differential Diagnostic Reagent for Detection of Mycobacterium bovis in Cattle{triangledown}

P. J. Cockle,* S. V. Gordon, R. G. Hewinson, and H. M. Vordermeier

TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone KT15 3NB, United Kingdom

Received 6 June 2006/ Returned for modification 7 July 2006/ Accepted 9 August 2006

In the search for improved tools with which to control bovine tuberculosis, the development of enhanced immunodiagnostic reagents is a high priority. Such reagents are required to improve the performance of tuberculin-based reagents and allow the discrimination of vaccinated cattle from those infected with Mycobacterium bovis. In this study, we identified the immunodominant, frequently recognized peptides from Rv3873, Rv3879c, Rv0288, and Rv3019c, which, together with peptides comprising the current lead diagnostic antigens, ESAT-6 and CFP-10, were formulated into a peptide cocktail. In a test of naturally infected cattle, this cocktail was significantly better than tuberculin was for identifying skin test-negative animals with confirmed bovine tuberculosis. In addition, the specificity of this cocktail was not compromised by Mycobacterium bovis BCG vaccination. In summary, our results prioritize this peptide-based, fully synthetic reagent for assessment in larger trials.

* Corresponding author. Mailing address: TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone KT15 3NB, United Kingdom. Phone: 44 1932 357 768. Fax: 44 1932 357 684. E-mail: p.cockle{at}vla.defra.gsi.gov.uk.

{triangledown} Published ahead of print on 30 August 2006.

Clinical and Vaccine Immunology, October 2006, p. 1119-1124, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00209-06




This article has been cited by other articles:

  • Schiller, I., Vordermeier, H. M., Waters, W. R., Palmer, M., Thacker, T., Whelan, A., Hardegger, R., Marg-Haufe, B., Raeber, A., Oesch, B. (2009). Assessment of Mycobacterium tuberculosis OmpATb as a Novel Antigen for the Diagnosis of Bovine Tuberculosis. CVI 16: 1314-1321 [Abstract] [Full Text]  
  • Meikle, V., Alito, A., Llera, A. S., Gioffre, A., Peralta, A., Buddle, B. M., Cataldi, A. (2009). Identification of Novel Mycobacterium bovis Antigens by Dissection of Crude Protein Fractions. CVI 16: 1352-1359 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»