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Clinical and Vaccine Immunology, January 2006, p. 116-122, Vol. 13, No. 1
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.1.116-122.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mycoplasma suis Antigens Recognized during Humoral Immune Response in Experimentally Infected Pigs

L. E. Hoelzle,1* K. Hoelzle,1 M. Ritzmann,2 K. Heinritzi,2 and M. M. Wittenbrink1

Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland,1 Clinic for Swine, Ludwig-Maximilians-University Munich, Munich, Germany2

Received 31 August 2005/ Returned for modification 7 October 2005/ Accepted 18 October 2005

Today, serodiagnostic tests for Mycoplasma suis infections in pigs have low accuracies. The development of novel serodiagnostic strategies requires a detailed analysis of the humoral immune response elicited by M. suis and, in particular, the identification of antigenic proteins of the agent. For this study, indirect enzyme-linked immunosorbent assay (ELISA) and immunoblot analyses were performed using pre- and sequential postinoculation sera from M. suis-infected and mock-infected control pigs. M. suis purified from porcine blood served as the antigen. Eight M. suis-specific antigens (p33, p40, p45, p57, p61, p70, p73, and p83) were identified as targets of the immunoglobulin G (IgG) antibody response during experimental infection, with p40, p45, and p70 being the preferentially recognized M. suis antigens. Besides the M. suis-specific antigens, porcine immunoglobulins were identified in blood-derived M. suis preparations. By immunoglobulin depletion, the specificity of the M. suis antigen for use in indirect ELISA was significantly improved. M. suis-specific Western blot and ELISA reactions were observed in all infected pigs by 14 days postinfection at the latest and until week 14, the end of the experiments. During acute clinical attacks of eperythrozoonosis, a derailment of the antibody response, determined by decreases in both the M. suis net ELISA values and the numbers of M. suis-specific immunoblot bands, was accompanied by peaking levels of autoreactive IgG antibodies. In conclusion, the M. suis-specific antigens found to stimulate specific IgG antibodies are potentially useful for the development of novel serodiagnostic tests.

* Corresponding author. Present address: Institute of Veterinary Bacteriology, University of Zurich, Winterthurerstrasse 270, 8057 Zurich, Switzerland. Phone: 41-1-635 8613. Fax: 41-1-635 8912. E-mail: lhoelzle{at}vetbakt.unizh.ch.

Clinical and Vaccine Immunology, January 2006, p. 116-122, Vol. 13, No. 1
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.1.116-122.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hoelzle, K., Grimm, J., Ritzmann, M., Heinritzi, K., Torgerson, P., Hamburger, A., Wittenbrink, M. M., Hoelzle, L. E. (2007). Use of Recombinant Antigens To Detect Antibodies against Mycoplasma suis, with Correlation of Serological Results to Hematological Findings. CVI 14: 1616-1622 [Abstract] [Full Text]  


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