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Clinical and Diagnostic Laboratory Immunology, August 2005, p. 1006-1009, Vol. 12, No. 8
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.8.1006-1009.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Microcapillary Cytometry Technology and Flow Cytometry for CD4+ and CD8+ T-Cell Estimation

A. J. Kandathil,1 R. Kannangai,1 S. David,1 G. Nithyanandam,1 S. Solomon,2 P. Balakrishnan,2 O. C. Abraham,3 S. Subramanian,3 P. Rupali,3 V. P. Verghese,4 S. Pulimood,5 and G. Sridharan1*

Department of Clinical Virology,1 Internal Medicine Unit 1,3 Child Health Unit 1,4 Department of Dermatology, Christian Medical College, Vellore, Tamil Nadu, India,5 VHS- YRG Care Infectious Diseases Laboratory, Taramani, Chennai, Tamil Nadu, India2

Received 30 March 2005/ Returned for modification 5 May 2005/ Accepted 16 May 2005

An alternative technology for the estimation of T cells based on a microcapillary technique (Guava Technologies, Hayward, CA) was compared to FACSCount (Becton Dickinson, San Jose, CA). Samples from 51 human immunodeficiency virus-infected and 21 healthy individuals were tested. The correlation (r) of the two systems for CD4+ T cells was 0.994, and the coefficient of variation was 6.5%, establishing equable performance between the two technologies.

* Corresponding author. Mailing address: Department of Clinical Virology, Christian Medical College, Vellore 632004, India. Phone: 91(416)2282070. Fax: 91(416)2232035 E-mail: g_sridharan{at}yahoo.com.

Clinical and Diagnostic Laboratory Immunology, August 2005, p. 1006-1009, Vol. 12, No. 8
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.8.1006-1009.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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