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Clinical and Diagnostic Laboratory Immunology, July 2005, p. 877-881, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.877-881.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Enzyme-Linked Immunosorbent Assay Based on a Chimeric Antigen Bearing Antigenic Regions of Structural Proteins Erns and E2 for Serodiagnosis of Classical Swine Fever Virus Infection

Min Lin,* Erin Trottier, and Maria Mallory

Animal Diseases Research Institute, Ottawa, Ontario, Canada K2H 8P9

Received 31 December 2004/ Returned for modification 2 February 2005/ Accepted 5 April 2005

The antigenic region (residues 109 to 160) of classical swine fever virus (CSFV) protein Erns and the N-terminal antigenic region (residues 1 to 136) of protein E2 were constructed in the form of a fused, chimeric protein, C21ErnsE2, for use as an enzyme-linked immunosorbent assay (ELISA) antigen for the serodiagnosis of CSFV infection. Tested with 238 negative-field (CSFV-free) sera from Canadian sources, the specificity of the ELISA was determined to be 93.7%. All 20 sera from experimentally infected pigs representing a variety of animals, virus strains, and days postinfection (dpi; range, 7 to 210) were detected as positive (100%). In contrast, an ELISA based on an Erns fragment (Ernsaa 109-160) or an E2 fragment (E2aa 1-221) identified only 18 (90%) of 20 sera from infected pigs as positive, missing two targets collected at 7 dpi. These data suggest that use of the chimeric antigen C21ErnsE2 would improve serodiagnostic sensitivity and allow for the detection of CSFV infection as early as 7 dpi.

* Corresponding author. Mailing address: Canadian Food Inspection Agency, Animal Diseases Research Institute, 3851 Fallowfield Road, Ottawa, Ontario, Canada K2H 8P9. Phone: (613) 228-6698. Fax: (613) 228-6667. E-mail: linm{at}inspection.gc.ca.

Clinical and Diagnostic Laboratory Immunology, July 2005, p. 877-881, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.877-881.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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