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Clinical and Diagnostic Laboratory Immunology, April 2005, p. 555-556, Vol. 12, No. 4
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.4.555-556.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Rapid Immunofluorescence Microscopy for Diagnosis of Melioidosis

Vanaporn Wuthiekanun,1* Varunee Desakorn,2 Gumphol Wongsuvan,1 Premjit Amornchai,1 Allen C. Cheng,1,3 Bina Maharjan,1 Direk Limmathurotsakul,1 Wirongrong Chierakul,1,2 Nicholas J. White,1,4 Nicholas P. J. Day,1,4 and Sharon J. Peacock1,4

Faculty of Tropical Medicine, Wellcome Trust-Oxford University-Mahidol University Tropical Medicine Research Program,1 Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand,2 Menzies School of Health Research, Charles Darwin University, and Northern Territory Clinical School, Flinders University, Darwin, Australia,3 Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom4

Received 26 November 2004/ Returned for modification 4 January 2005/ Accepted 14 January 2005

An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively.

* Corresponding author. Mailing address: Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Rd., Bangkok 10400, Thailand. Phone: 66 2 354 9172. Fax: 66 2 354 9169. E-mail: lek{at}tropmedres.ac.

Clinical and Diagnostic Laboratory Immunology, April 2005, p. 555-556, Vol. 12, No. 4
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.4.555-556.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Novak, R. T., Glass, M. B., Gee, J. E., Gal, D., Mayo, M. J., Currie, B. J., Wilkins, P. P. (2006). Development and Evaluation of a Real-Time PCR Assay Targeting the Type III Secretion System of Burkholderia pseudomallei. J. Clin. Microbiol. 44: 85-90 [Abstract] [Full Text]  
  • Peacock, S. J., Chieng, G., Cheng, A. C., Dance, D. A. B., Amornchai, P., Wongsuvan, G., Teerawattanasook, N., Chierakul, W., Day, N. P. J., Wuthiekanun, V. (2005). Comparison of Ashdown's Medium, Burkholderia cepacia Medium, and Burkholderia pseudomallei Selective Agar for Clinical Isolation of Burkholderia pseudomallei. J. Clin. Microbiol. 43: 5359-5361 [Abstract] [Full Text]  
  • VELD, D. H. I. T, WUTHIEKANUN, V., CHENG, A. C., CHIERAKUL, W., CHAOWAGUL, W., BROUWER, A. E., WHITE, N. J., DAY, N. P. J., PEACOCK, S. J. (2005). THE ROLE AND SIGNIFICANCE OF SPUTUM CULTURES IN THE DIAGNOSIS OF MELIOIDOSIS. Am J Trop Med Hyg 73: 657-661 [Abstract] [Full Text]  


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