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Clinical and Diagnostic Laboratory Immunology, March 2005, p. 474-476, Vol. 12, No. 3
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.3.474-476.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of the COOH Portion of the Nucleocapsid Protein in an Antigen-Capturing Enzyme-Linked Immunosorbent Assay for Specific and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus

Maofeng Qiu,1 Jin Wang,1 Hongxia Wang,1 Zeliang Chen,1 Erhei Dai,1 Zhaobiao Guo,1 Xiaoyi Wang,1 Xin Pang,1 Baoxing Fan,2 Jie Wen,3 Jian Wang,3 and Ruifu Yang1*

Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences,1 PLA General Hospital,2 Beijing Genomics Institute, Chinese Academy of Sciences, Beijing, People's Republic of China3

Received 26 November 2004/ Returned for modification 28 December 2004/ Accepted 5 January 2005

Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.

* Corresponding author. Mailing address: Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, People's Republic of China. Phone: 86-10-66948595. Fax: 86-10-83820748. E-mail: yangrf{at}nic.bmi.ac.cn.

Clinical and Diagnostic Laboratory Immunology, March 2005, p. 474-476, Vol. 12, No. 3
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.3.474-476.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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Copyright © 2005 by the American Society for Microbiology. All rights reserved.