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Clinical and Diagnostic Laboratory Immunology, February 2005, p. 347-353, Vol. 12, No. 2
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.2.347-353.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Long-Term Infection of Mongolian Gerbils with Helicobacter pylori: Microbiological, Histopathological, and Serological Analyses

Shigehito Nakagawa,1,2 Takako Osaki,1 Yasunori Fujioka,3 Hiroyuki Yamaguchi,4 and Shigeru Kamiya1*

Department of Infectious Diseases,1 Department of Pathology, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo,3 Division of Molecular Microbiology and Biochemistry, Department of Basic Laboratory Sciences, School of Allied Health Sciences, Osaka University, Yamadaoka, Suita, Osaka,4 Aventis Pharma Ltd., Minami-dai, Kawagoe, Saitama, Japan2

Received 26 July 2004/ Returned for modification 19 August 2004/ Accepted 21 October 2004

The effects of long-term infection with Helicobacter pylori on the gastric mucosa of Mongolian gerbils were examined. Colonization by H. pylori was evaluated by both microaerobic cultivation and real-time reverse transcriptase PCR (RT-PCR). Persistent infection with H. pylori in gastric mucosa was detected by real-time RT-PCR during 6 months after infection, but no H. pylori was isolated 4 months after infection by cultivation. Infiltration with neutrophils and mononuclear cells was observed from 2 months after infection. Both intestinal metaplasia and gastric atrophy were also detected from 2 months after infection. The results by enzyme-linked immunosorbent assay indicated that antibody titers against whole H. pylori antigens, H. pylori heat shock protein 60 (HSP60), and Escherichia coli GroEL were significantly higher in the infected gerbils than in noninfected gerbils. After long-term infection with H. pylori for 18 months, marked atrophy of gastric mucosa and multiple cysts in the submucosa were observed in the glandular stomach of the infected gerbils. In addition, squamous cell papilloma with hyperkeratosis was observed in cardia of all the infected gerbils. These results indicate that evaluation of bacterial colonization during long-term infection can be done by real-time RT-PCR and that mucosal damage might be induced by host immune response against whole H. pylori antigen.

* Corresponding author. Mailing address: Department of Microbiology, Division of Infectious Diseases, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan. Phone: 81-422-47-5511, ext. 3462. Fax: 81-422-44-7325. E-mail: skamiya{at}kyorin-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, February 2005, p. 347-353, Vol. 12, No. 2
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.2.347-353.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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