This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kjos, S. A.
Right arrow Articles by Chappell, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kjos, S. A.
Right arrow Articles by Chappell, C. L.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, February 2005, p. 268-272, Vol. 12, No. 2
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.2.268-272.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of Recombinant Oocyst Protein CP41 for Detection of Cryptosporidium-Specific Antibodies

Sonia A. Kjos,1,{dagger} Mark Jenkins,2 Pablo C. Okhuysen,1,3 and Cynthia L. Chappell1*

Center for Infectious Diseases, The University of Texas Health Science Center School of Public Health,1 Division of Infectious Diseases, The University of Texas Health Science Center Medical School, Houston, Texas,3 Immunology and Disease Resistance Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland2

Received 12 October 2004/ Returned for modification 12 November 2004/ Accepted 13 December 2004

Cryptosporidium is an important cause of diarrhea in developed and developing countries, and its epidemiology is of interest. The methodologies used in the detection of Cryptosporidium-specific antibodies vary widely, which complicates comparison of results. This study assesses the performance of a Cryptosporidium recombinant protein (rCP41) in a serological assay compared to that of a crude antigen preparation. The 41-kDa protein from the oocyst wall was previously cloned and expressed in Escherichia coli. Sera from 192 healthy adults from the Texas Medical Center (Houston) were tested for anti-Cryptosporidium antibody reactivity using both crude and recombinant antigen preparations in an enzyme-linked immunosorbent assay. Immunoglobulin G reactivity was highly concordant (88%; P < 0.0001) between the two antigen preparations, with 110 positive (57%) and 59 negative (31%) by both tests. Regression analysis revealed a high correlation between the absorbance values generated with both antigen preparations and suggests that the rCP41 may be used in place of crude antigen. These results indicate that the use of the recombinant CP41 antigen in a standardized serodiagnostic assay could provide a reliable and cost-effective method for assessing human exposure to Cryptosporidium.

* Corresponding author. Mailing address: University of Texas School of Public Health, 1200 Herman Pressler Dr., Ste 118A, Houston, TX 77030. Phone: (713) 500-9026. Fax: (713) 500-9020. E-mail: Cynthia.L.Chappell{at}uth.tmc.edu.

{dagger} Present address: Department of Entomology, Texas A&M University, College Station, TX 77843-2475.

Clinical and Diagnostic Laboratory Immunology, February 2005, p. 268-272, Vol. 12, No. 2
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.2.268-272.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»