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Clinical and Diagnostic Laboratory Immunology, December 2005, p. 1451-1454, Vol. 12, No. 12
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.12.1451-1454.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Immunoglobulin E Binding Reactivity of a Recombinant Allergen Homologous to {alpha}-Tubulin from Tyrophagus putrescentiae

Kyoung Yong Jeong,1 Haeseok Lee,1 Jae Sik Lee,1 Jongweon Lee,1 In-Yong Lee,1 Han-Il Ree,1 Chein-Soo Hong,2 Jung Won Park,2 and Tai-Soon Yong1*

Department of Parasitology and Institute of Tropical Medicine,1 Department of Internal Medicine and Institute of Allergy, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea2

Received 17 April 2005/ Returned for modification 11 July 2005/ Accepted 5 August 2005

Storage mites may cause allergic respiratory diseases in urban areas as well as pose an occupational hazard in rural areas. Characterization of storage mite allergens is important for the development of diagnostic and therapeutic agents against mite-associated allergic disorders. Here we report on the cloning and expression of {alpha}-tubulin from the storage mite (Tyrophagus putrescentiae). The deduced amino acid sequence of the {alpha}-tubulin from the storage mite showed as much as 97.3% identity to the {alpha}-tubulin sequences from other organisms. The highly conserved amino acid sequences of {alpha}-tubulins across different species of mites may indicate that cross-reactivity for this potential allergen exists. The frequency of immunoglobulin E reactivity of this recombinant protein is 29.3% in sera from storage mite-allergic subjects.

* Corresponding author. Mailing address: Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea. Phone: 82-2-228-1840. Fax: 82-2-363-8676. E-mail: tsyong212{at}yumc.yonsei.ac.kr.

Clinical and Diagnostic Laboratory Immunology, December 2005, p. 1451-1454, Vol. 12, No. 12
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.12.1451-1454.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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