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Clinical and Diagnostic Laboratory Immunology, November 2005, p. 1343-1346, Vol. 12, No. 11
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.11.1343-1346.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection of Babesia canis rossi, B. canis vogeli, and Hepatozoon canis in Dogs in a Village of Eastern Sudan by Using a Screening PCR and Sequencing Methodologies

Maremichi Oyamada,1 Bernard Davoust,2 Mickaël Boni,3 Jacques Dereure,4 Bruno Bucheton,5 Awad Hammad,6 Kazuhito Itamoto,1 Masaru Okuda,1 and Hisashi Inokuma7*

Faculty of Agriculture, Yamaguchi University, 753-8515 Yamaguchi, Japan,1 Direction du Service de Santé en Region Sud-Est, BP16, 69998 Lyon Armees, France,2 Groupe de Secteurs Vétérinaires Interarmees de Saint Germain en Laye, BP220, 00492 Saint Germain en Laye Armees, France,3 Laboratoire de Parasitologie, Faculté de Médecine, 34090 Montpellier, France,4 Laboratoire d'immunologie Parasitaire, Faculté de Médecine, Université de la Méditerranée, Marseille Cedex 5, France,5 Departement of Microbiology and Parasitology, Faculty of Medecine, University of Khartoum, P.O. Box 102, Khartoum, Republic of the Sudan,6 Obihiro University of Agriculture and Veterinary Medicine, 080-8555 Obihiro, Japan7

Received 13 July 2005/ Returned for modification 18 August 2005/ Accepted 19 August 2005

Babesia and Hepatozoon infections of dogs in a village of eastern Sudan were analyzed by using a single PCR and sequencing. Among 78 dogs, 5 were infected with Babesia canis rossi and 2 others were infected with B. canis vogeli. Thirty-three dogs were positive for Hepatozoon. Hepatozoon canis was detected by sequence analysis.

* Corresponding author. Mailing address: Obihiro University of Agriculture and Veterinary Medicine, 080-8555 Obihiro, Japan. Phone and fax: (81)-155-49-5370. E-mail: inokuma{at}obihiro.ac.jp.

Clinical and Diagnostic Laboratory Immunology, November 2005, p. 1343-1346, Vol. 12, No. 11
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.11.1343-1346.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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