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Clinical and Diagnostic Laboratory Immunology, October 2005, p. 1231-1234, Vol. 12, No. 10
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.10.1231-1234.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of LIAISON Treponema Screen, a Novel Recombinant Antigen-Based Chemiluminescence Immunoassay for Laboratory Diagnosis of Syphilis

Antonella Marangoni,1 Vittorio Sambri,1* Silvia Accardo,1 Francesca Cavrini,2 Antonietta D'Antuono,3 Alessandra Moroni,1 Elisa Storni,2 and Roberto Cevenini1

Microbiology Section,1 Dermatology Section, DMCSS, University of Bologna,3 Centro di Riferimento Regionale per le Emergenze Microbiologiche, Ospedale Policlinico S. Orsola, Bologna, Italy2

Received 30 May 2005/ Returned for modification 22 June 2005/ Accepted 29 July 2005

The purpose of this study was to evaluate the diagnostic performance of LIAISON Treponema Screen (DiaSorin, Saluggia, Italy), a new automated chemiluminescence immunoassay (CLIA), in comparison with that of rapid plasma reagin (RPR) and the following currently used treponemal tests: hemagglutination test (TPHA), immunoenzymatic assay (EIA), and Western blot (WB). First, a retrospective study was performed with a panel of 2,494 blood donor sera, a panel of 131 clinical and serologically characterized syphilitic sera, and 96 samples obtained from subjects with potentially interfering diseases or conditions. A prospective study was also performed by testing 1,800 unselected samples submitted to the Microbiology Laboratory of the St. Orsola Hospital in Bologna, Italy, for routine screening for syphilis. As expected, RPR was the least specific method, especially when potentially cross-reacting sera were tested. On the contrary, all of the treponemal tests proved to be very specific (99.9%) and they performed with the following sensitivities: 100% (WB), 99.2% (CLIA), 95.4% (EIA), and 94.7% (TPHA).

* Corresponding author. Mailing address: Section of Microbiology, DMCSS, University of Bologna, St. Orsola Hospital, via Massarenti 9, 40138 Bologna, Italy. Phone: 39 051 4290 913. Fax: 39 051 636 4516. E-mail: vsambri{at}med.unibo.it.

Clinical and Diagnostic Laboratory Immunology, October 2005, p. 1231-1234, Vol. 12, No. 10
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.10.1231-1234.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Young, H, Pryde, J, Duncan, L, Dave, J (2009). The Architect Syphilis assay for antibodies to Treponema pallidum: an automated screening assay with high sensitivity in primary syphilis. Sex. Transm. Infect. 85: 19-23 [Abstract] [Full Text]  


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