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Clinical and Diagnostic Laboratory Immunology, January 2005, p. 187-191, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.187-191.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Expression of Recombinant Small Hydrophobic Protein for Serospecific Detection of Avian Pneumovirus Subgroup C

Lizhong Luo,1* Marta I. Sabara,1 and Yan Li2

Canadian Food Inspection Agency, National Centre for Foreign Animal Disease,2 National Microbiology Laboratory, Canadian Science Center for Human and Animal Health, Winnipeg, Manitoba, Canada1

Received 25 June 2004/ Returned for modification 22 September 2004/ Accepted 4 October 2004

The small hydrophobic (SH) gene of the avian pneumovirus (APV) Colorado isolate (CO), which belongs to subgroup C (APV/C), was expressed with a baculovirus vector. The recombinant SH protein was evaluated as a potential subgroup-specific diagnostic reagent in order to differentiate infections resulting from APV/C from those induced by APV/A, APV/B, and human metapneumovirus (hMPV). When the recombinant baculovirus was used to infect insect cells, a 31- to 38-kDa glycosylated form of the SH protein was produced and subsequently tested for reactivity with antibodies specific for APV/A, APV/B, APV/C, and hMPV. Western blot analysis showed that the expressed recombinant SH protein could only be recognized by APV/C-specific antibodies. This result was consistent with sequence analysis of the APV/C SH protein, which had very low (24%) amino acid identity with the corresponding protein of hMPV and no discernible identity with the SH protein of APV/A or APV/B. A recombinant SH protein-based enzyme-linked immunosorbent assay (ELISA) was developed, and it further confirmed the lack of reactivity of this protein with antisera raised to APV/A, APV/B, and hMPV and supported its designation as a subgroup-specific antigen. This finding indicated that the recombinant SH protein was a suitable antigen for ELISA-based detection of subgroup-specific antibodies in turkeys and could be used for serologically based differential diagnosis of APV and hMPV infections.

* Corresponding author. Mailing address: Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba R3E 3M4, Canada. Phone: (204) 789-2149. Fax: (204) 789-2038. E-mail: luol{at}inspection.gc.ca.

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 187-191, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.187-191.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Luo, L., Sabara, M. I. (2005). Production of a Recombinant Major Inner Capsid Protein for Serological Detection of Epizootic Hemorrhagic Disease Virus. CVI 12: 904-909 [Abstract] [Full Text]  


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