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Clinical and Diagnostic Laboratory Immunology, January 2005, p. 122-124, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.122-124.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Another B-Cell Epitope in the Type-Specific Region of Equine Herpesvirus 4 Glycoprotein G

Ken Maeda,1* Fuminori Mizukoshi,1 Masataka Hamano,1 Kazushige Kai,1 Takashi Kondo,2 and Tomio Matsumura2

Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi,1 Epizootic Research Station, Equine Research Institute, Japan Racing Association, Shiba, Kokubunji-Machi, Shimotsuga-gun, Tochigi, Japan2

Received 19 July 2004/ Returned for modification 28 September 2004/ Accepted 26 October 2004

Recently, a novel 12-mer B-cell epitope, MKNNPIYSEGSL, in the type-specific region of equine herpesvirus 1 (EHV-1) glycoprotein G (gG) was identified and used as an antigen for enzyme-linked immunosorbent assay (Maeda et al., J. Clin. Microbiol. 42:1095-1098, 2004). Although our prototype strain, TH20p, possesses two repeat sequences containing the B-cell epitope, the EHV-4 NS80567 strain has two repeat sequences that are not identical. One repeat sequence stretch contained the B-cell epitope, while the other contained the 11-mer, MKNNPVYSESL (underlining indicates a different amino acid). In this study, heterogeneity of the type-specific region was compared among Japanese EHV-4 isolates. The 11-mer peptide, MKNNPVYSESL, specifically reacted with sera from horses naturally infected with EHV-4 but not with sera from horses experimentally infected with EHV-4 TH20p. The 11-mer peptide may be another B-cell epitope in the type-specific region.

* Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. Phone and fax: 81-83-933-5887. E-mail: kmaeda{at}yamaguchi-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 122-124, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.122-124.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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