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Clinical and Diagnostic Laboratory Immunology, January 2005, p. 114-121, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.114-121.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Antigenic and Immunogenic Investigation of B-Cell Epitopes in the Nucleocapsid Protein of Peste des Petits Ruminants Virus

Kang-Seuk Choi,1* Jin-Ju Nah,1 Young-Joon Ko,1 Shien-Young Kang,2 Kyoung-Jin Yoon,3 and Nam-In Jo1

Foreign Animal Disease Division, National Veterinary Research and Quarantine Service, Anyang, Kyoung-gi,1 College of Veterinary Medicine, Chungbuk National University, Heungduk-Ku, Cheongju, Chungbuk, Korea,2 Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa3

Received 11 July 2004/ Returned for modification 28 September 2004/ Accepted 22 October 2004

Attempts were made to identify and map epitopes on the nucleocapsid (N) protein of peste des petits ruminants virus (PPRV) (Nigeria75/1 strain) using seven monoclonal antibodies (MAbs) and deletion mutants. At least four antigenic domains (A-I, A-II, C-I, and C-II) were identified using the MAbs. Domains A-I (MAb 33-4) and A-II (MAbs 38-4, P-3H12, and P-13A9) were determined to be located on the amino-terminal half (amino acids [aa] 1 to 262), and domains C-I (P-14C6) and C-II (P-9H10 and P-11A6) were within the carboxy-terminal region (aa 448 to 521). Nonreciprocal competition between A-II MAbs and MAbs to C-I and C-II domains was observed, indicating that they may be exposed on the surface of the N protein and spatially overlap each other. Blocking or competitive enzyme-linked immunosorbent assay studies using PPRV serum antibodies revealed that epitopes on the domains A-II and C-II were immunodominant, whereas those on the domains A-I and C-I were not. The competition between MAb and rinderpest virus (RPV) serum antibodies raised against RPV strain LATC was found in two epitopes (P-3H12 and P-13A9) on the domain A-II, indicating that these epitopes may cause cross-reactivity between PPRV and RPV. Identification of immunodominant but PPRV-specific epitopes and domains will provide the foundation in designing an N-protein-based diagnostic immunoassay for PPRV.

* Corresponding author. Mailing address: Foreign Animal Disease Division, National Veterinary Research and Quarantine Service, 480 Anyang-6 dong, Anyang, Kyoung-gi, 430-824, Korea. Phone: 82-31-467-1860. Fax: 82-31-449-5882. E-mail: choiks{at}nvrqs.go.kr.

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 114-121, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.114-121.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Choi, K.-S., Nah, J.-J., Ko, Y.-J., Kang, S.-Y., Jo, N.-I. (2005). Rapid Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Peste des Petits Ruminants Virus. CVI 12: 542-547 [Abstract] [Full Text]  


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