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Clinical and Diagnostic Laboratory Immunology, November 2004, p. 1165-1170, Vol. 11, No. 6
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.6.1165-1170.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Utility of Schistosoma bovis Adult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques

J. Pardo,1 C. Carranza,2 M. C. Turrientes,3 J. L. Pérez Arellano,2 R. López Vélez,3 V. Ramajo,4 and A. Muro1*

Laboratorio de Inmunología Parasitaria y Molecular, CISET, Facultad de Farmacia, Universidad de Salamanca,1 Unidad de Patología Animal, Instituto de Recursos Naturales y Agrobiología, CSIC, Salamanca,4 Departamento de Ciencias Médicas y Quirúrgicas, Facultad de Ciencias de la Salud, Universidad de Las Palmas de Gran Canaria, Las Palmas de Gran Canaria,2 Medicina Tropical y Parasitologia Clinica, Servicio de Enfermedades Infecciosas, Hospital Ramón y Cajal, Madrid, Spain3

Received 11 May 2004/ Returned for modification 3 August 2004/ Accepted 8 September 2004

Immunodiagnostic methods based on the detection of antibodies continue to be the most effective and practical methods for the diagnosis of imported schistosomiasis. Schistosoma bovis is a species whose final natural hosts are bovines, ovines, caprines, and small wild ruminants. Different studies have demonstrated the analogies existing between S. bovis and other Schistosoma species which affect humans. The objective of this work was to evaluate the utility of S. bovis adult worm antigens (AWA) for the diagnosis of imported human schistosomiasis by enzyme-linked immunosorbent assay (ELISA) and electroimmunotransfer blotting (EITB) techniques. By detecting eggs, the ELISA for S. bovis AWA was able to definitively detect imported cases with a sensitivity of 94%. The specificity of the ELISA for S. bovis AWA was 97%. There were no differences between the results of the S. bovis AWA ELISA for patients infected with Schistosoma mansoni and those infected with Schistosoma haematobium. The EITB technique showed bands of 85, 37, and 20 kDa, which are characteristic of infections with Schistosoma spp. Specific bands to indicate infection by different species of Schistosoma have not been detected. The combined use of the ELISA for S. bovis AWA and EITB increased the global sensitivity of the study to 97%. Our findings suggest that the ELISA for S. bovis AWA is a useful test for the immunodiagnosis of imported schistosomiasis and that EITB for detecting S. bovis AWA permits the confirmation of diagnosis when the ELISA for S. bovis AWA is positive.

* Corresponding author. Mailing address: Laboratorio de Inmunología Parasitaria y Molecular, CISET, Facultad de Farmacia, Universidad de Salamanca, Avda. Campo Charro s/n 37007, Salamanca, Spain. Phone: 34923294535. Fax: 34923294515. E-mail: ama{at}usal.es.

Clinical and Diagnostic Laboratory Immunology, November 2004, p. 1165-1170, Vol. 11, No. 6
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.6.1165-1170.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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