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Clinical and Diagnostic Laboratory Immunology, November 2004, p. 1022-1027, Vol. 11, No. 6
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.6.1022-1027.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of Antibodies in Lymphocyte Secretions for Detection of Subclinical Tuberculosis Infection in Asymptomatic Contacts

Rubhana Raqib,1* S. M. Mostafa Kamal,2 M. Jubayer Rahman,1 Zeaur Rahim,1 Sayera Banu,1 Pradip K. Bardhan,1 Fahima Chowdhury,1 Gul Ara,1 K. Zaman,1 Robert F. Breiman,1 Jan Andersson,3 and David A. Sack1

ICDDR,B-Centre for Health and Population Research,1 Pathology and Microbiology Department, National Institute of Diseases of the Chest and Hospital, Dhaka, Bangladesh,2 Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden3

Received 2 February 2004/ Returned for modification 29 April 2004/ Accepted 22 July 2004

We have previously demonstrated that Mycobacterium bovis BCG-specific immunoglobulin G antibodies in lymphocyte secretions (ALS) can be employed as a marker for active tuberculosis (TB). We aimed to determine whether the ALS method allows detection of subclinical TB infection in asymptomatic individuals. A prospective study of family contacts (FCs) of patients with active TB and healthy controls was performed. Thirteen of 42 FCs had high ALS responses, including 6 FCs who subsequently developed active TB. No correlation was observed between the tuberculin skin test and the ALS responses in the FCs (r = 0.1, P = 0.23). Among patients with active TB, BCG-specific ALS responses steadily declined from the time of diagnosis through 6 months following antimycobacterial chemotherapy (P = 0.001). The ALS assay enabled detection of infection in exposed symptom-free contacts, who are at greater risk for developing active TB. The method may also allow discrimination between effective treatment of active infection and suboptimal response to therapy.

* Corresponding author. Mailing address: Immunology Laboratory, Laboratory Sciences Division, ICDDR,B: International Center for Health & Population Research, Mohakhali, Dhaka-1212, Bangladesh. Phone: 880-2 8811751, ext. 2404. Fax: 880-2 8812529. E-mail: rubhana{at}icddrb.org.

Clinical and Diagnostic Laboratory Immunology, November 2004, p. 1022-1027, Vol. 11, No. 6
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.6.1022-1027.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kirkpatrick, B. D., Bentley, M. D., Thern, A. M., Larsson, C. J., Ventrone, C., Sreenivasan, M. V., Bourgeois, L. (2005). Comparison of the Antibodies in Lymphocyte Supernatant and Antibody-Secreting Cell Assays for Measuring Intestinal Mucosal Immune Response to a Novel Oral Typhoid Vaccine (M01ZH09). CVI 12: 1127-1129 [Abstract] [Full Text]  


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