This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Soethout, E. C.
Right arrow Articles by Rutten, V. P. M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Soethout, E. C.
Right arrow Articles by Rutten, V. P. M. G.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, July 2004, p. 795-798, Vol. 11, No. 4
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.4.795-798.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification and Phenotyping of Leukocytes in Bovine Bronchoalveolar Lavage Fluid

E. C. Soethout,1,2* K. E. Müller,2 A. J. M. van den Belt,3 and V. P. M. G. Rutten1

Division of Immunology, Department of Infectious Diseases and Immunology,1 Department of Farm Animal Health,2 Division of Diagnostic Imaging, Faculty of Veterinary Medicine, Utrecht University, 3508 TD Utrecht, The Netherlands3

Received 29 July 2003/ Returned for modification 8 October 2003/ Accepted 2 February 2004

A method is proposed to identify leukocyte subpopulations in bovine bronchoalveolar lavage fluid by dual-laser flow cytometry. The technique uses several parameters, i.e., exclusion of highly autofluorescent alveolar macrophages and inclusion of leukocytes on the basis of labeling by specific antibodies and light scatter characteristics.

* Corresponding author. Present address: Nederlands Vaccin Instituut, Department of LVR, Internal Post Box 92, Post Box 457, 3720 AL, Bilthoven, The Netherlands. Phone: 31 30 2744001. Fax: 31 30 2744429. E-mail: ernst.soethout{at}nvi-vaccin.nl.

Clinical and Diagnostic Laboratory Immunology, July 2004, p. 795-798, Vol. 11, No. 4
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.4.795-798.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»