This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Waters, W. R.
Right arrow Articles by Minion, F. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Waters, W. R.
Right arrow Articles by Minion, F. C.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, July 2004, p. 729-735, Vol. 11, No. 4
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.4.729-735.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of Recombinant ESAT-6:CFP-10 Fusion Protein for Differentiation of Infections of Cattle by Mycobacterium bovis and by M. avium subsp. avium and M. avium subsp. paratuberculosis

W. R. Waters,1* B. J. Nonnecke,2 M. V. Palmer,1 S. Robbe-Austermann,1 J. P. Bannantine,1 J. R. Stabel,1 D. L. Whipple,1 J. B. Payeur,3 D. M. Estes,4,5 J. E. Pitzer,6 and F. C. Minion6

Bacterial Diseases of Livestock Research Unit,1 Periparturient Diseases of Cattle Research Unit, National Animal Disease Center, Agricultural Research Service,2 Mycobacteria and Brucella Section, National Veterinary Services Laboratory, Veterinary Services, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, Iowa 50010,3 Department of Pediatrics,4 Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas 77555,5 Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 500116

Received 10 February 2004/ Returned for modification 18 March 2004/ Accepted 27 March 2004

Immunological diagnosis of Mycobacterium bovis infection of cattle is often confounded by cross-reactive responses resulting from exposure to other mycobacterial species, especially Mycobacterium avium. Early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) are dominant gamma interferon (IFN-{gamma})-inducing antigens of tuberculous mycobacteria, and they are absent from many environmental nontuberculous mycobacteria. Because M. avium exposure is the primary confounding factor in the diagnosis of M. bovis-infected animals, in vitro responses to a recombinant ESAT-6:CFP-10 (rESAT-6:CFP-10) fusion protein by blood leukocytes from cattle naturally exposed to M. avium or experimentally challenged with Mycobacterium avium subsp. avium or Mycobacterium avium subsp. paratuberculosis were compared to responses by M. bovis-infected cattle. Responses to heterogeneous mycobacterial antigens (i.e., purified protein derivatives [PPDs] and whole-cell sonicates [WCSs]) were also evaluated. Tumor necrosis factor alpha (TNF-{alpha}), IFN-{gamma}, and nitric oxide responses by M. bovis-infected cattle to rESAT-6:CFP-10 exceeded (P < 0.05) the corresponding responses by cattle naturally sensitized to M. avium. Experimental infection with M. bovis, M. avium, or M. avium subsp. paratuberculosis induced significant (P < 0.05) IFN-{gamma} and nitric oxide production to WCS and PPD antigens, regardless of the mycobacterial species used for the preparation of the antigen. Responses to homologous crude antigens generally exceeded responses to heterologous antigens. Nitric oxide and IFN-{gamma} responses to rESAT-6:CFP-10 by blood leukocytes from M. bovis-infected calves exceeded (P < 0.05) the corresponding responses of noninfected, M. avium-infected, and M. avium subsp. paratuberculosis-infected calves. Despite the reported potential for secretion of immunogenic ESAT-6 and CFP-10 proteins by M. avium and M. avium subsp. paratuberculosis, it appears that use of the rESAT-6:CFP-10 fusion protein will be useful for the detection of tuberculous cattle in herds with pre-existing sensitization to M. avium and/or M. avium subsp. paratuberculosis.

* Corresponding author. Mailing address: National Animal Disease Center, P.O. Box 70, Ames, IA 50010-0070. Phone: (515) 663-7756. Fax: (515) 663-7458. E-mail: rwaters{at}nadc.ars.usda.gov.

Clinical and Diagnostic Laboratory Immunology, July 2004, p. 729-735, Vol. 11, No. 4
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.4.729-735.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Green, L. R., Jones, C. C., Sherwood, A. L., Garkavi, I. V., Cangelosi, G. A., Thacker, T. C., Palmer, M. V., Waters, W. R., Rathe, C. V. (2009). Single-Antigen Serological Testing for Bovine Tuberculosis. CVI 16: 1309-1313 [Abstract] [Full Text]  
  • Waters, W. R., Palmer, M. V., Slaughter, R. E., Jones, S. L., Pitzer, J. E., Minion, F. C. (2006). Diagnostic Implications of Antigen-Induced Gamma Interferon Production by Blood Leukocytes from Mycobacterium bovis-Infected Reindeer (Rangifer tarandus). CVI 13: 37-44 [Abstract] [Full Text]  
  • Maue, A. C., Waters, W. R., Davis, W. C., Palmer, M. V., Minion, F. C., Estes, D. M. (2005). Analysis of Immune Responses Directed toward a Recombinant Early Secretory Antigenic Target Six-Kilodalton Protein-Culture Filtrate Protein 10 Fusion Protein in Mycobacterium bovis-Infected Cattle. Infect. Immun. 73: 6659-6667 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»