This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nájera, O.
Right arrow Articles by Ortiz, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nájera, O.
Right arrow Articles by Ortiz, R.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, May 2004, p. 577-580, Vol. 11, No. 3
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.3.577-580.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Flow Cytometry Study of Lymphocyte Subsets in Malnourished and Well-Nourished Children with Bacterial Infections

Oralia Nájera,1* Cristina González,2 Guadalupe Toledo,3 Laura López,4 and Rocío Ortiz2

Departamento de Atención a la Salud Universidad Autónoma Metropolitana—Xochimilco,1 Hospital Materno-Pediátrico, Xochimilco,1 Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana—Iztapalapa,2 Secretaría de Salud del Gobierno del Distrito Federal, Iztapalapa, México4

Received 19 November 2003/ Returned for modification 6 January 2004/ Accepted 9 March 2004

Protein-energy malnutrition is the primary cause of immune deficiency in children across the world. It has been related to changes in peripheral T-lymphocyte subsets. The aim of the present study was to evaluate the effects of infection and malnutrition on the proportion of peripheral-lymphocyte subsets in well-nourished non-bacterium-infected (WN), well-nourished bacterium-infected (WNI), and malnourished bacterium-infected (MNI) children by flow cytometry. A prospectively monitored cohort of 15 MNI, 12 WNI, and 17 WN children was studied. All the children were 3 years old or younger and had only bacterial infections. Results showed a significant decrease in the proportion of T CD3+ (P < 0.05 for relative and P < 0.03 for absolute values), CD4+ (P < 0.01 for relative and absolute values), and CD8+ (P < 0.05 for relative values) lymphocyte subsets in WNI children compared to the results seen with WN children. Additionally, B lymphocytes in MNI children showed significant lower values (CD20+ P < 0.02 for relative and P < 0.05 for absolute values) in relation to the results seen with WNI children. These results suggest that the decreased proportions of T-lymphocyte subsets observed in WNI children were associated with infection diseases and that the incapacity to increase the proportion of B lymphocyte was associated with malnutrition. This low proportion of B lymphocytes may be associated with the mechanisms involved in the immunodeficiency of malnourished children.

* Corresponding author. Mailing address: Universidad Autónoma Metropolitana—Xochimilco. Departamento de Atención a la Salud, Calz. del Hueso 1100, Col. Villa Quietud, A. P. 23-181, Coyoacán, 04960 México, D. F. México. Phone: 54 83 72 45. Fax: 54 83 72 18. E-mail: onajera{at}cueyatl.uam.mx.

Clinical and Diagnostic Laboratory Immunology, May 2004, p. 577-580, Vol. 11, No. 3
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.3.577-580.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rodriguez, L., Gonzalez, C., Flores, L., Jimenez-Zamudio, L., Graniel, J., Ortiz, R. (2005). Assessment by Flow Cytometry of Cytokine Production in Malnourished Children. CVI 12: 502-507 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»