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Clinical and Diagnostic Laboratory Immunology, March 2004, p. 430-432, Vol. 11, No. 2
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.2.430-432.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Performance Characteristics of Updated INNO-LiPA Assays for Molecular Typing of Human Leukocyte Antigen A (HLA-A), HLA-B, and HLA-DQB1 Alleles

Karen De Vreese,1* Rachel Barylski,2 Fiona Pughe,2 Miriam Bläser,1 Colin Evans,2 John Norton,2 Gilbert Semana,3 Richard Holman,4 Pascale Loiseau,5 Dominique Masson,6 Mieke Gielis,7 Annelies De Brauwer,1 Ilse De Canck,1 Gonda Verpooten,1 and Frank Hulstaert1

Innogenetics N.V., B-9052 Ghent,1 Antwerp Blood Transfusion Center, Edegem, Belgium,7 Abbott-Murex Ltd., Dartford, Kent,2 The Anthony Nolan Trust, Royal Free Hospital, London, United Kingdom,4 Etablissement de Transfusion Sanguine, Rennes,3 Service d'Immunologie et d'Histocompatibilité, Hôpital Saint Louis, Paris,5 Etablissement de Transfusion Sanguine, La Tronche, France6

Received 13 June 2003/ Returned for modification 8 August 2003/ Accepted 3 December 2003

We carried out a multicenter performance evaluation of three new DNA-based human leukocyte antigen (HLA) typing assays: INNO-LiPA HLA-A Update, INNO-LiPA HLA-B Update, and INNO-LiPA HLA-DQB1 Update. After optimization, the accuracy rates were all 100%, and the final observed resolutions were 99.4, 92.4, and 85.6%, respectively. These rapid and easy-to-perform assays yielded results fully concordant with other DNA-based tissue typing tests.

* Corresponding author. Mailing address: Innogenetics N.V., Technologiepark 6, B-9052 Ghent, Belgium. Phone: 32 9 329 1735. Fax: 32 9 329 1768. E-mail: Karen_De_Vreese{at}innogenetics.be.

Clinical and Diagnostic Laboratory Immunology, March 2004, p. 430-432, Vol. 11, No. 2
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.2.430-432.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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