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Clinical and Diagnostic Laboratory Immunology, March 2004, p. 239-244, Vol. 11, No. 2
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.2.239-244.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Depressed Type 1 Cytokine Synthesis by Superantigen-Activated CD4+ T Cells of Women with Human Papillomavirus-Related High-Grade Squamous Intraepithelial Lesions

Bang-Ning Lee,1* Michele Follen,2,3 De-Yu Shen,1 Anais Malpica,4 Karen Adler-Storthz,5,6 William T. Shearer,7 and James M. Reuben1

Departments of Hematopathology,1 Gynecologic Oncology,2 Pathology, The University of Texas M.D. Anderson Cancer Center,4 Dental Branch,5 Department of Obstetrics, Gynecology, and Reproductive Sciences,3 Graduate School of Biomedical Sciences, The University of Texas Health Science Center,6 Department of Pediatrics, Baylor College of Medicine, Houston, Texas7

Received 16 April 2003/ Returned for modification 11 July 2003/ Accepted 31 December 2003

Carcinoma of the cervix is causally related to infection with the human papillomavirus (HPV), and T cells play a pivotal role in the immune response of the host to rid itself of HPV infection. Therefore, we assessed the T-cell function of women with HPV-related cervical neoplasia against a superantigen, Staphylococcus enterotoxin B (SEB). Each woman provided a cervical brush specimen for HPV DNA testing and Papanicolaou (Pap) smears for the staging of cervical lesions. They also provided a blood specimen for determination of the ability of CD4+ T and CD8+ T cells to synthesize Th1 (interleukin-2 [IL-2], gamma interferon [IFN-{gamma}], and tumor necrosis factor alpha [TNF-{alpha}]) and Th2 (IL-10) cytokines in response to activation with SEB. Compared with control subjects with self-attested negative Pap smears, women with high-grade squamous intraepithelial lesions (HSIL) had significantly lower percentages of activated CD4+ T cells that produced IL-2 (P = 0.045), IFN-{gamma} (P = 0.040), and TNF-{alpha} (P = 0.015) and a significantly lower percentage of activated CD8+ T cells that produced IL-2 (P < 0.01). These data indicate that women with HPV-related cervical HSIL show a decrease in Th1 cytokine production by activated CD4+ T cells and suggested that compromised T-helper functions may negatively impact the function of cytotoxic CD8+ T cells.

* Corresponding author. Mailing address: Department of Hematopathology/Unit 54, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030. Phone: (713) 792-3394. Fax: (713) 792-4296. E-mail: blee{at}mdanderson.org.

Clinical and Diagnostic Laboratory Immunology, March 2004, p. 239-244, Vol. 11, No. 2
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.2.239-244.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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