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Clinical and Diagnostic Laboratory Immunology, January 2004, p. 227-228, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.227-228.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Profile of Antibodies to the Nucleocapsid Protein of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Probable SARS Patients

Xuan Liu,1 Yulin Shi,2 Ping Li,1 Linhai Li,2 Yanping Yi,1 Qingjun Ma,1 and Cheng Cao1*

Beijing Institute of Biotechnology, Beijing 100850,1 General Hospital of Guangzhou Command of PLA, Guangzhou 510010, Peoples Republic of China2

Received 25 August 2003/ Returned for modification 28 October 2003/ Accepted 5 November 2003

Profiles of antibodies to the nucleocapsid protein of the severe acute respiratory syndrome (SARS)-associated coronavirus in 445 probable SARS patients and 3,749 healthy people or non-SARS patients were analyzed by antigen-capturing enzyme-linked immunosorbent assay. Antinucleocapsid antibodies were elucidated in 17.5% of the probable SARS patients 1 to 7 days after the onset of symptoms and in 80% of the patients 8 to 14 days after the onset. About 90% of the probable SARS patients were positive 15 or more days after illness. Antibody titers increased up to 70 days, and high antibody titers were maintained at least for another 3 months. Of the healthy people and non-SARS patients, only seven (0.187%) were weakly positive.

* Corresponding author. Mailing address: Beijing Institute of Biotechnology, P.O. Box 130(8), 27 Taiping Rd., Beijing 100850, Peoples Republic of China. Phone and fax: 86 10 68155151. E-mail: caoc{at}nic.bmi.ac.cn.

Clinical and Diagnostic Laboratory Immunology, January 2004, p. 227-228, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.227-228.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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