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Clinical and Diagnostic Laboratory Immunology, January 2004, p. 111-114, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.111-114.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Antibody Reactivity to Omp31 from Brucella melitensis in Human and Animal Infections by Smooth and Rough Brucellae

Juliana Cassataro,1 Karina Pasquevich,1 Laura Bruno,1 Jorge C. Wallach,2,3 Carlos A. Fossati,2 and Pablo C. Baldi2*

Laboratorio de Inmunogenética, Hospital de Clínicas,1 Instituto de Estudios de la Inmunidad Humoral, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires,2 Servicio de Brucelosis, Hospital F.J. Muñiz, Buenos Aires, Argentina3

Received 23 September 2003/ Returned for modification 27 October 2003/ Accepted 7 November 2003

Group 3 of outer membrane proteins (OMPs) of Brucella includes Omp25 and Omp31, which share 34% identity. Omp25 is highly conserved in Brucella species, and Omp31 is present in all Brucella species, except Brucella abortus. Antibodies to Brucella melitensis Omp31 have been sought only in infected sheep, and Western blotting of sera from infected sheep did not reveal anti-Omp31 reactivity. We obtained recombinant purified Omp31 (B. melitensis) and tested its recognition by sera from humans and animals suffering from brucellosis by an indirect enzyme-linked immunosorbent assay (ELISA). Serum samples from 74 patients, 57 sheep, and 47 dogs were analyzed; brucellosis was confirmed by bacteriological isolation in all ovine and canine cases and 31 human cases of brucellosis. Thirty-five patients (47%) were positive for antibodies to Omp31, including seven cases of Brucella suis infection, two cases of B. abortus infection, and three cases of B. melitensis infection. Of 39 sheep naturally infected with B. melitensis (biovars 1 and 3), 23 (59%) were positive for antibodies to Omp31. Anti-Omp31 antibodies were also detected in 12 of 18 rams (67%) in which Brucella ovis was isolated from semen. Antibodies to Omp31 were also found in 41 (87%) of the 47 dogs, including 13 with recent infection. These results suggest that an indirect ELISA using recombinant purified Omp31 from B. melitensis would be of limited value for the diagnosis of human and animal brucellosis. Nevertheless, the potential usefulness of this antigen in combination with other recombinant proteins from Brucella should not be dismissed.

* Corresponding author. Mailing address: Instituto de Estudios de la Inmunidad Humoral, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 4to. piso, 1113 Buenos Aires, Argentina. Phone: 54-11-4964-8259. Fax: 54-11-4964-0024. E-mail: pablobal{at}ffyb.uba.ar.

Clinical and Diagnostic Laboratory Immunology, January 2004, p. 111-114, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.111-114.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cassataro, J., Estein, S. M., Pasquevich, K. A., Velikovsky, C. A., de la Barrera, S., Bowden, R., Fossati, C. A., Giambartolomei, G. H. (2005). Vaccination with the Recombinant Brucella Outer Membrane Protein 31 or a Derived 27-Amino-Acid Synthetic Peptide Elicits a CD4+ T Helper 1 Response That Protects against Brucella melitensis Infection. Infect. Immun. 73: 8079-8088 [Abstract] [Full Text]  
  • Cassataro, J., Velikovsky, C. A., de la Barrera, S., Estein, S. M., Bruno, L., Bowden, R., Pasquevich, K. A., Fossati, C. A., Giambartolomei, G. H. (2005). A DNA Vaccine Coding for the Brucella Outer Membrane Protein 31 Confers Protection against B. melitensis and B. ovis Infection by Eliciting a Specific Cytotoxic Response. Infect. Immun. 73: 6537-6546 [Abstract] [Full Text]  


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