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Clinical and Diagnostic Laboratory Immunology, July 1999, p. 479-482, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Serological Diagnosis of Human Cysticercosis by Use of Recombinant Antigens from Taenia solium Cysticerci

Kerstin Hubert,1 Abel Andriantsimahavandy,2 Alain Michault,3 Matthias Frosch,1 and Fritz A. Mühlschlegel1,*

Institut für Hygiene und Mikrobiologie, Universität Würzburg, 97080 Würzburg, Germany1; Parasitology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar2; and Department of Parasitology, Regional Hospital of Saint-Pierre, La Réunion3

Received 11 November 1998/Returned for modification 10 February 1999/Accepted 18 March 1999

A Taenia solium metacestode cDNA expression library in the lambda ZAPII vector was screened with pooled sera from patients with neurocysticercosis. Sixty primary clones were identified and shown to belong to two classes. The clones NC-3 and NC-9 did not reveal any significant homologies to sequences deposited in the databases and were further characterized. Both recombinant antigens were expressed as glutathione S-transferase fusion proteins and applied for serological diagnosis of human cysticercosis. An enzyme-linked immunosorbent assay was established and evaluated with 27 serum samples of La Réunion and Madagascar patients with cysticercosis. Diagnosis in these patients was established with radiological and serological procedures. For antigen NC-3 a sensitivity of 96.3% and a specificity of 91.5% for the serodiagnosis were achieved. In contrast, the sensitivity of antigen NC-9 was only 33.3%.

* Corresponding author. Mailing address: Institut für Hygiene und Mikrobiologie, Universitat Würzburg, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany. Phone: (0931) 201-3931 Fax: (0931) 201-3445. E-mail: fmuehlschlegel{at}hygiene.uni-wuerzburg.de.

Clinical and Diagnostic Laboratory Immunology, July 1999, p. 479-482, Vol. 6, No. 4
1071-412X/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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